Surface Plasmon Resonance (SPR) Service
Surface Plasmon Resonance (SPR) technology is a leading and standard-setting method in the field of biomolecular interactions. SPR provides unique insights into the interactions between proteins and other biomolecules, enabling scientists to gain a deeper understanding of biomolecular functions and make better-informed decisions to enhance productivity.
Through SPR, you can accurately measure:
• Association rate (ka) – how fast molecules come together
• Dissociation rate (kd) – how fast molecules separate
• Affinity constant (KD) – the strength of the interaction
SPR enables deep insights into binding kinetics for:
• Antibody Screening, Characterization, Consistency evaluation
• Biomolecules interactions
• Small molecule affinity testing
• Fc receptors proteins/complement affinity assay
How SPR Works?
Figure Origin: Nguyen HH, Park J, Kang S, Kim M. Surface plasmon resonance: a versatile technique for biosensor applications. Sensors (Basel). 2015;15(5):10481-10510. Published 2015 May 5.
Step 1: Ligand Immobilization
Ligands are immobilized on a sensor chip surface using direct coupling or capture strategies. Pilot runs ensure stable attachment (20–50 RU typical response).
Step 2: Analyte Injection
Analytes flow over the immobilized ligand. SPR detects real-time refractive index changes near the sensor surface, proportional to mass accumulation.
Step 3: Signal Response and Regeneration
Each binding event generates a sensorgram—a real-time signal curve.
After measurement, the chip is regenerated to remove bound analyte while maintaining ligand activity, allowing multi-cycle analysis.
Step 4: Data Evaluation
Binding kinetics are analyzed by comparing response levels across concentrations to determine affinity (KD) and specificity.
Service Platform
Biacore 8K
Our SPR services are powered by the Biacore 8K platform, providing:
Real-time kinetic analysis with industry-leading speed and sensitivity
Compatibility with a wide range of molecular sizes and types
Service Advantages
| Feature | Advantage | Benefit |
|---|---|---|
| 5-day turnaround for ≤5 test pairs | Rapid project completion | Accelerate decision-making |
|
Free ACRO protein (Exclude transmembrane protein and nanodisc products) |
No additional reagent cost | Reduced total project costs and expenses |
| ISO/IEC 17025 accreditation | Meets international quality and traceability standards | Data accepted by regulatory agencies |
| Cytiva Biacore 8K | High-speed, high-accuracy measurements | Reliable kinetic profiles |
| FDA-recognized method | Regulatory compliance | Supports IND and BLA submissions |
| Lab in Delaware | Reduced shipping time | Time-saving service |
SPR Service Inquiry
Interested in evaluating your biomolecular interactions with our SPR platform?
Complete the inquiry form below to discuss your project needs with our technical specialists, we’ll get back to you with a proposal and timeline shortly.
Case Study
Membrane Protein Interaction Analysis
Anti-CD37 antibody (Naratuximab) captured on Protein A Chip can bind Human CD37 Full Length Protein, His,Flag Tag (Cat. No. CD7-H52D3) with an affinity constant of 20.3 nM as determined in a SPR assay (in presence of DDM and CHS) (Biacore 8K).
Claudin-18 Monoclonal Antibody captured on CM5 chip via anti-mouse antibodies surface can bind Mouse Claudin-18.2, His,Twin-Strep Tag (Cat. No. CL2-M5585) with an affinity constant of 13 nM as determined in a SPR assay (in presence of DDM and CHS) (Biacore 8K) .
Anti-Claudin-6 Antibody, Human IgG1 captured on Protein A Chip can bind Human Claudin-6 Protein, His,Twin-Strep Tag (Cat. No. CL6-H5289) with an affinity constant of 4.95 nM as determined in a SPR assay (Biacore 8K).
Extracellular Matrix Interaction Study
Immune Complex Recognition and Affinity Measurement
Anti-NY-ESO-1 Antibody, Human IgG1 captured on Protein A Chip can bind Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQV) Tetramer Protein (Cat. No. HL1-H52C6) with an affinity constant of 0.752 nM as determined in a SPR assay (Biacore 8K) .
NY-ESO-1 TCR captured on Protein A Chip can bind Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQV) Tetramer Protein (Cat. No. HL1-H52E7) with an affinity constant of 15.7 nM as determined in a SPR assay (Biacore 8K) .
Service Workflow
Certification
In December 2024，ACROBiosystems’ US Innovation Lab has achieved ISO/IEC 17025 accreditation for Surface Plasmon Resonance (SPR) analytical service, ensuring that results from this service is globally recognized and trusted for its precision and consistency.
- Overview
- How SPR Works?
- Service Platform
- Service Advantages
- SPR Service Inquiry
- Case Study
- Service Workflow
- Certification