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APC-Labeled Monoclonal Anti-Human CD73 Antibody, Mouse IgG1 (5A10) (0.03% Proclin)

COADS MS
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FABm024-02-100tests (50tests X 2)
$220.00
ETA of in-stock products:2 business days
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Product Details

  • Source

    Monoclonal Anti-Human CD73 Antibody, Mouse IgG1 (5A10) is a mouse monoclonal antibody recombinantly expressed from Hybridoma.

  • Antibody Type

    Hybridoma Monoclonal

  • Clone

    5A10

  • Isotype

    Mouse Fc, Kappa

  • Host Species

    Mouse

  • Reactivity

    Human

  • Immunogen

    Purified Human CD73 Protein

  • Conjugate

    APC

    Excitation Wavelength: 640 nm

    Emission Wavelength: 661 nm

  • Specificity

    This product is a specific antibody specifically reacts with CD73 protein.

  • Application

    Flow Cytometry (Evaluation of the expression of CD73 on Human cells).

  • Recommended Dilution

    1:20

  • Form

    Liquid

  • Formulation

    Supplied as 0.2 μm-filtered solution in PBS (pH 7.4) containing 0.03% ProClin 300 and 0.2% BSA, with trehalose as protectant.

    Please contact us for customized product forms or formulations.

  • Shipping

    Liquid product is shipped at blue ice.

  • Storage

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. Store at 2-8 °C for 12 months.

  • Notices

    Product Specific Notices: For research use only.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • Bioactivity-FACS

    CD73 FACS

    Flow cytometric analysis of MSC staining with APC-Labeled Monoclonal Anti-Human CD73 Antibody, Mouse IgG1 (5A10) (Cat. No. FABm024-02) at 1:20 dilution (5 μL of the antibody stock solution corresponds to labeling of 1e6 cells in a final volume of 100 µL), compared with isotype control antibody. APC signal was used to evaluate the binding activity (QC tested).

    Protocol

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Background

5'-nucleotidase (5'-NT), also known as ecto-5'-nucleotidase or CD73 (cluster of differentiation 73), is an enzyme that is encoded by the NT5E gene. CD73 commonly serves to convert AMP to adenosine. Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. Rare allelic variants are associated with a syndrome of adult-onset calcification of joints and arteries (CALJA) affecting the iliac, femoral, and tibial arteries reducing circulation in the legs and the joints of the hands and feet causing pain.

Recent Advances

 
Drug Development Progress
  • English Name：

    5'-nucleotidase

  • Category：

  • Approved Drugs：

    0 Details

  • Drugs in Clinical Trials：

    36 Details

  • Highest Development Stage：

    Phase 3 Clinical

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