PE-Labeled Monoclonal Anti-HLA class I Antibody, Mouse IgG2a (W632) (0.03% Proclin)

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Cat. No. / Size
Price
Qty
HLI-PCFM816-25tests
$60.00
HLI-PCFM816-200tests
$330.00
ETA of in-stock products:2 business days

Product Details

  • Source

    PE-Labeled Monoclonal Anti-HLA class I Antibody, Mouse IgG2a (W632) is a Mouse monoclonal antibody recombinantly expressed from HEK293 cells, which provides higher batch consistency and long term security of supply.

  • Antibody Type

    Recombinant Monoclonal

  • Clone

    W6/32

  • Isotype

    Mouse IgG2a, Kappa

  • Host Species

    Mouse

  • Reactivity

    Human

  • Immunogen

    Purified HLA class I Protein

  • Conjugate

    PE

    Excitation Wavelength: 488 nm / 561 nm

    Emission Wavelength: 575 nm

  • Specificity

    This product is a specific antibody specifically reacts with HLA class I protein.

  • Application

    Flow Cytometry (Evaluation of the expression of HLA class I on Human cells).

  • Recommended Dilution

    1:50

  • Isotype Control

    The isotype control is sold separately; please refer to Cat. No. DNP-PM487 for product information.

  • Form

    Lyophilized

  • Formulation

    Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4) containing 0.03% ProClin 300 and 0.2% BSA, with trehalose as protectant.

    Please contact us for customized product forms or formulations.

  • Reconstitution

    Please refer to the Certificate of Analysis (CoA) for specific instructions.

  • Shipping

    Lyophilized product is shipped at ambient temperature.

  • Storage

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 24 months in lyophilized state;
    2. -70°C for 12 months after reconstitution.
    3. 2-8 °C for 12 months after reconstitution.
  • Notices

    Product Specific Notices: For research use only.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • Bioactivity-FACS

     HLA class I FACS

    Flow cytometric analysis of Human peripheral blood lymphocytes staining with PE-Labeled Monoclonal Anti-HLA class I Antibody, Mouse IgG2a (W632) (Cat. No. HLI-PCFM816) at 1:50 dilution (2 μL of the antibody stock solution corresponds to labeling of 1e6 PBMCs in a final volume of 100 µL), compared with PE Mouse IgG2a, k lsotype Ctrl(FC) Antibody. PE signal was used to evaluate the binding activity (QC tested).

    Protocol

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Background

The W6/32 monoclonal antibody reacts with the human major histocompatibility complex (MHC) class I, HLA-A, B, C. MHC class I antigens associated with beta 2-microglobulin are expressed by all human nucleated cells and are central in cell-mediated immune response and tumor surveillance. W6/32 mAb recognizes a non-polymorphic epitope shared among products of the HLA-A, B, and C loci and immunoprecipitates both 43 kDa and 11-12 kDa chains. Crossreactivity is also seen in baboon, rhesus and cynomolgus monkey.

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