FITC-Labeled Mouse IgG2a Antibody Isotype Control

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Cat. No. / Size
Price
Qty
DNP-FM487-25tests
$50.00
DNP-FM487-200tests
$205.00
ETA of in-stock products:2 business days

Product Details

  • Source

    Mouse IgG2a Antibody Isotype Control is a monoclonal antibody recombinantly expressed from human 293 cells (HEK293), which provides higher batch consistency and long term security of supply.

  • Antibody Type

    Recombinant Monoclonal

  • Isotype

    Mouse IgG2a, Kappa

  • Host Species

    Mouse

  • Immunogen

    DNP (Dinitrophenyl)

  • Conjugate

    FITC

    Excitation source: 488 nm spectral line, argon-ion laser

    Excitation Wavelength: 488 nm

    Emission Wavelength: 535 nm

  • Specificity

    Specifically reacts with DNP (Dinitrophenyl) and DNP conjugated proteins, which is normally not present in Humans or animals. Therefore, DNP-FM487 does not react with any antigen of Human or animal cells.

  • Application

    Flow Cytometry.

  • Recommended Dilution

    1:50

  • Form

    Lyophilized

  • Formulation

    Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4) containing 0.03% ProClin 300, with trehalose as protectant.

    Please contact us for customized product forms or formulations.

  • Reconstitution

    Please refer to the Certificate of Analysis (CoA) for specific instructions.

  • Shipping

    Lyophilized product is shipped at ambient temperature.

  • Storage

    For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 24 months in lyophilized state;
    2. -70°C for 12 months after reconstitution;
    3. 2-8°C for 12 months after reconstitution.
  • Notices

    Product Specific Notices: For research use only.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • Bioactivity-FACS

     IgG control FACS

    5e5 of PBMCs were stained with FITC-Labeled Mouse IgG2a Antibody Isotype Control (Cat. No. DNP-FM487), washed and then analyzed with FACS. FITC signal was used to evaluate the non-specific binding activity to PBMCs (QC tested).

    Protocol

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Background

A hapten is a small molecule that can elicit an immune response only when conjugated with a large carrier such as a protein. Typical haptens include drugs, urushiol, quinone, steroids, etc. Peptides and non-protein antigens usually need conjugating to a carrier protein (such as BSA (bovine serum albumin) or KLH (keyhole limpet hemocyanin) to become good immunogens). Additionally, haptens should be administered with an adjuvant to ensure a high quality immune response. It is important that the hapten design (preserving greatly the chemical structure and spatial conformation of target compound), selection of the appropriate carrier protein and the conjugation method are key conditions for the desired specificity anti-hapten antibodies. We design anti-hapten antibodies based on the HaptenDB information.

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