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Détails du produit
Product Overview
Human IFN-alpha-2 ELISA Kit ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human IFN-alpha-2 that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ELISA Kit provides several benefits:
- Standards to calibrate with NIBSC/WHO standards for comparable results.
- Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
- High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
- Simplified and straightforward protocols and ready-to-use reagents to save assay time.
Product Specifications
Assay TypeSandwich-ELISAAnalyteIFN-α2Format96-wells plate breakable into 12 x 8 wells stripsReactivityHumanSensitivity150 pg/mLAssay Time1.75 hrSample volume50 μLRange312.5 pg/mL-20000 pg/mLSample TypeCell Culture Supernatants, Plasma, Serum.NIBSC Code94/784Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
Application
The kit is developed for quantitative detection of human IFN alpha 2 in serum, plasma and cell culture supernates.
It is for research use only.
Workflow
Storage
Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Materials Provided
IDComponentsSizeCEA240-C01Pre-coated Anti-IFN-α2 Antibody Microplate1 plate (8×12 strips)CEA240-C02Human IFN-α2 Standard200 ng×2CEA240-C03Biotin-Anti-IFN-α2 Antibody Con. Solution100μLCEA240-C04Biotin-Antibody Dilution Buffer8 mLCEA240-C05Streptavidin-HRP Con. Solution500 μLCEA240-C06Streptavidin-HRP Dilution Buffer15 mLCEA240-C0720×Washing Buffer50 mLCEA240-C08Sample Dilution Buffer15 mL×2CEA240-C09Substrate Solution12 mLCEA240-C10Stop Solution6 mLACRO Quality Management System
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Typical Data
Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was cal-culated based on the results of the standard curve. The minimum detectable con-centration of IFN-α2 is less than 150 pg/mL.
Sample Values
Cell Culture Supernatant: Human peripheral blood mononuclear cells (5 x 10⁶ cells/mL) were cultured in RPMI 1640 supplemented with 10% fetal bovine serum, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Cells were untreated or treated with 10 ug/mL of poly I:C in the presence of Lipofectamine 2000 for 24 hours. Subse-quent analysis involved quantitative measurement of IFN-α2 concentrations in collected culture supernatants using standardized immunoassays.
Intra-Assay Statistics
Ten replicates of each of three samples containing different IFN-α2 concentrations were tested in one assay. Acceptable criteria: CV＜10%.
Inter-Assay Statistics
Three samples containing different concentrations of IFN-α2 were tested in inde-pendent assays. Acceptable criteria: CV<15%.
Recovery
Recombinant IFN-α2 was spiked into three human serum samples, and then analyzed. The average recovery of IFN-α2 for serum samples is 103.6%.
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