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Détails du produit
Synonyms
TNFRSF9, 4-1BB, CD137, CDw137, ILA
Source
Human 4-1BB, His Tag (41B-H52Hc) is expressed from human 293 cells (HEK293). It contains AA Leu 24 - Gln 186 (Accession # Q07011-1).
Predicted N-terminus: Leu 24
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 19.2 kDa. The protein migrates as 27-35 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Affichage des données
SDS-PAGE
Human 4-1BB, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human 4-1BB, His Tag (Cat. No. 41B-H52Hc) is more than 95% and the molecular weight of this protein is around 20-30 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human 4-1BB, His Tag (Cat. No. 41B-H52Hc) at 2 μg/mL (100 μL/well) can bind Human 4-1BB Ligand (71-254), Fc Tag, active trimer with a linear range of 0.1-0.8 ng/mL (Routinely tested).
Protocol
Bioactivity-SPR
Biotinylated Human 4-1BB Ligand (71-254), Fc,Avitag, premium grade (Cat. No. 41L-H82Fa) captured on Protein A Chip can bind Human 4-1BB, His Tag (Cat. No. 41B-H52Hc) with an affinity constant of 342 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Avis des utilisateurs Publier un commentaire
유***진2026-04-21
J'aimeI purchased ACRO’s Human 4-1BB (His Tag) for our recent binding affinity studies. The most impressive aspect was the provided MALS (Multi-Angle Light Scattering) data, which gave us high confidence in the protein's oligomeric state and purity before even starting our experiments. Since 4-1BB function is highly dependent on its structural integrity, having MALS-verified molecular weight data is a huge advantage. We observed excellent consistency in our SPR (Surface Plasmon Resonance) assays with minimal lot-to-lot variation. Highly recommended for researchers requiring high-precision analytical data.- M***i2023-03-21 J'aimeI have ordered this material several times for use in Biacore/SPR assays. I have been able to obtain consistent, high-quality results with different lots of this antigen and was able to reproduce kinetic measurements reported in literature for commercial anti-4-1BB molecules. Because 4-1BB is glycosylated, the molecular weight reported by SEC-MALS is helpful for estimating Rmax for my experiments. We have also used this antigen to successfully capture anti-4-1BB out of serum in ELISAs.
Introduction de fond
Progrès de frontière
Progrès du développement médicamenteux- Nom anglais：
Tumor necrosis factor receptor superfamily member 9
- Catégorie：
- Nombre de médicaments répertoriés：
0 Détails
- Nombre de médicaments cliniques：
77 Détails
- Étape de développement la plus élevée：
Phase 3 Clinical
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