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Détails du produit
Synonyms
BAFFR, TNFRSF13C, BROMIX, CD268, CVID4, prolixin, BAFF-R
Source
Human BAFFR Protein, Llama IgG2b Fc Tag (BAR-H5258) is expressed from human 293 cells (HEK293). It contains AA Ser 7 - Ala 71 (Accession # Q96RJ3-1).
Predicted N-terminus: Ser 7
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a llama IgG2b Fc tag at the C-terminus.
The protein has a calculated MW of 34.4 kDa. The protein migrates as 42-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Affichage des données
SDS-PAGE
Human BAFFR Protein, Llama IgG2b Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-ELISA
Immobilized Human BAFF, His Tag at 2 μg/mL (100 μL/well) can bind Human BAFFR, Llama IgG2b Fc Tag (Cat. No. BAR-H5258) with a linear range of 2-125 ng/mL (QC tested).
Protocol
Immobilized Human BAFFR Protein, Llama IgG2b Fc Tag (Cat. No. BAR-H5258) at 2 μg/mL (100 μL/well) can bind Biotinylated Human BAFF, His,Avitag (Cat. No. BAF-H82Q2) with a linear range of 0.2-4 ng/mL (Routinely tested).
Protocol
Immobilized Human BAFFR Protein, Llama IgG2b Fc Tag (Cat. No. BAR-H5258) at 1 μg/mL (100 μL/well) can bind Anti-BAFFR Antibody, Human IgG1 with a linear range of 0.2-5 ng/mL (QC tested).
Protocol
Serial dilutions of Anti-BAFFR Antibody, Human IgG1 were added into Human BAFFR Protein, Llama IgG2b Fc Tag (Cat. No. BAR-H5258): Biotinylated Human BAFF Protein, His,Avitag (Cat. No. BAF-H82Q2) binding reactions. The half maximal inhibitory concentration (IC50) is 1.1323 μg/mL (Routinely tested).
Protocol
Bioactivity-SPR
Immobilized Human BAFF Protein, Fc Tag (Cat. No. BAF-H5261) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human BAFFR Protein, Llama IgG2b Fc Tag (Cat. No. BAR-H5258) with an affinity constant of 44.5 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
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Introduction de fond
Progrès de frontière
Progrès du développement médicamenteux- Nom anglais：
B-cell activating factor receptor
- Catégorie：
- Nombre de médicaments répertoriés：
0 Détails
- Nombre de médicaments cliniques：
14 Détails
- Étape de développement la plus élevée：
NDA/BLA
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