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Détails du produit
Synonyms
CD28, Tp44
Source
Human / Cynomolgus / Rhesus macaque CD28, Mouse IgG2a Fc Tag (CD8-H52A5) is expressed from human 293 cells (HEK293). It contains AA Asn 19 - Pro 152 (Accession # P10747-1). In the region Asn 19 - Pro 152, the amino acid sequences of Human, Cynomolgus and Rhesus macaque CD28 are identical.
Predicted N-terminus: Asn 19
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a mouse IgG2a Fc tag at the C-terminus.
The protein has a calculated MW of 42.0 kDa. The protein migrates as 50-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.1 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Affichage des données
SDS-PAGE
Human / Cynomolgus / Rhesus macaque CD28, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Human / Cynomolgus / Rhesus macaque CD28, Mouse IgG2a Fc Tag (Cat. No. CD8-H52A5) is more than 95% and the molecular weight of this protein is around 99-121 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human / Cynomolgus / Rhesus macaque CD28, Mouse IgG2a Fc Tag (Cat. No. CD8-H52A5) at 5 μg/mL (100 μL/well) can bind Human B7-2, Fc Tag (Cat. No. CD6-H5257) with a linear range of 0.1-5 μg/mL (QC tested).
Protocol
Bioactivity-BLI
Loaded Human / Cynomolgus / Rhesus macaque CD28, Mouse IgG2a Fc Tag (Cat. No. CD8-H52A5) on Protein A Biosensor, can bind Human B7-1, His Tag (Cat. No. B71-H5228) with an affinity constant of 1.82 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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Introduction de fond
Progrès de frontière
Progrès du développement médicamenteux- Nom anglais：
T-cell-specific surface glycoprotein CD28
- Catégorie：
- Nombre de médicaments répertoriés：
0 Détails
- Nombre de médicaments cliniques：
31 Détails
- Étape de développement la plus élevée：
Phase 2 Clinical
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