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Détails du produit
Synonyms
FLJ18683, T3E, TCRE, CD3E, CD3-epsilon
Source
Human CD3 epsilon, His Tag (CDE-H5223) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (Accession # P07766-1).
Predicted N-terminus: Asp 23
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 16.9 kDa. Maybe caused by unknown PTMs, the protein migrates as 18-19 kDa and 21-22 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition, and 35-43 kDa under non-reducing (NR) condition (SDS-PAGE).
The protein is designed as a dimer.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 24 months in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Affichage des données
SDS-PAGE
Human CD3 epsilon, His Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human CD3 epsilon, His Tag (Cat. No. CDE-H5223) is more than 90% and the molecular weight of this protein is around 38-55 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human CD3 epsilon, His Tag (Cat. No. CDE-H5223) at 1 μg/mL (100 μL/well) can bind Mouse Anti-Human CD3 (SP34-2) with a linear range of 0.2-3 ng/mL (QC tested).
Protocol
Immobilized Mouse Anti Human CD3(SP-34) at 2 μg/mL (100 μL/well) can bind Human CD3 epsilon, His Tag (Cat. No. CDE-H5223) with a linear range of 0.2-2 ng/mL (Routinely tested).
Protocol
Immobilized Human CD3 epsilon, His Tag (Cat. No. CDE-H5223) at 1 μg/mL (100 μL/well) can bind Anti-BCMA×CD3 Antibody (Teclistamab Biosimilar) with a linear range of 0.06-8 ng/mL (Routinely tested).
Protocol
Bioactivity-TR-FRET
In this TR-FRET assay, Monoclonal Anti-His Tag Antibody Europium chelate (for TR-FRET) (Cat. No. FRT-TD05) is used as the Donor and Streptavidin Protein FA-Labeled (for TR-FRET) (Cat. No. FRT-TA01) is used as the Acceptor. Human CD3 epsilon, His Tag (Cat. No. CDE-H5223), at 2.5 ng/well and Biotinylated Human BCMA, Fc,Avitag, premium grade (Cat. No. BC7-H82F0), at 2.5 ng/well, can bind to the Teclistamab with a linear detection range of 2-0.001 μg/mL (Routinely tested).
Bioactivity-SPR
Monoclonal Anti-Human CD3 Antibody, Mouse IgG1 (SP34-2) (Cat. No. CDE-M531) captured on CM5 chip via anti-mouse antibodies surface can bind Human CD3 epsilon, His Tag (Cat. No. CDE-H5223) with an affinity constant of 0.394 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Bispecific T-cell Engager captured on Protein A Chip can bind Human CD3 epsilon, His Tag (Cat. No. CDE-H5223) with an affinity constant of 0.843 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Anti-Human CD3 mAb, mouse IgG1 (Clone # SP34-2) on AMC Biosensor, can bind Human CD3 epsilon, His Tag (Cat. No. CDE-H5223) with an affinity constant of 0.341 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Bispecific T-cell Engager (CD3 X BCMA) on AHC Biosensor via DMF Filed Human BCMA, Fc Tag (Cat. No. BC7-H5254), can bind Human CD3 epsilon, His Tag (Cat. No. CDE-H5223) with an affinity constant of 0.147 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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Introduction de fond
Progrès de frontière
Progrès du développement médicamenteux- Nom anglais：
T-cell surface glycoprotein CD3 epsilon chain
- Catégorie：
- Nombre de médicaments répertoriés：
4 Détails
- Nombre de médicaments cliniques：
7 Détails
- Étape de développement la plus élevée：
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