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Product Details
Synonyms
FLJ18683, T3E, TCRE, CD3E, CD3-epsilon
Source
Human CD3 epsilon, Mouse IgG2a Fc Tag (CDN-H5253) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (Accession # NP_000724.1).
Predicted N-terminus: Asp 23
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a mouse IgG2a Fc tag at the C-terminus.
The protein has a calculated MW of 38.6 kDa. The protein migrates as 43-50 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human CD3 epsilon, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human CD3 epsilon, Mouse IgG2a Fc Tag (Cat. No. CDN-H5253) is more than 95% and the molecular weight of this protein is around 75-90 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human CD3 epsilon, Mouse IgG2a Fc Tag (Cat. No. CDN-H5253) at 1 μg/mL (100 μL/well) can bind Mouse Anti-Human CD3 (SP34-2) (mouse IgG1) with a linear range of 9.8-39 ng/mL (QC tested).
Protocol
Bioactivity-TR-FRET
In this TR-FRET assay, Monoclonal Anti-His Tag Antibody Europium chelate (for TR-FRET) (Cat. No. FRT-TD05) is used as the Donor and Monoclonal Anti-Mouse IgG Antibody FA-Labeled (for TR-FRET) (Cat. No. FRT-TA03) is used as the Acceptor. Human CD20 Full Length, His Tag (Cat. No. CD0-H52H1), at 5 ng/well and Human CD3 epsilon, Mouse IgG2a Fc Tag (Cat. No. CDN-H5253), at 2.5 ng/well, can bind to the Glofitamab with a linear detection range of 16-0.008 μg/mL (Routinely tested).
In this TR-FRET assay, Monoclonal Anti-His Tag Antibody Europium chelate (for TR-FRET) (Cat. No. FRT-TD05) is used as the Donor and Monoclonal Anti-Mouse IgG Antibody FA-Labeled (for TR-FRET) (Cat. No. FRT-TA03) is used as the Acceptor. Human DLL3, His Tag (Cat. No. DL3-H52H4), at 1.25 ng/well and Human CD3 epsilon, Mouse IgG2a Fc Tag (Cat. No. CDN-H5253), at 1.25 ng/well, can bind to the Tarlatamab with a linear detection range of 2-0.008 μg/mL (Routinely tested).
Bioactivity-SPR
Anti-Human CD3 mAb, mouse IgG1 (Clone # SP34-2) immobilized on CM5 Chip can bind Human CD3 epsilon, Mouse IgG2a Fc Tag (Cat. No. CDN-H5253) with an affinity constant of 75.2 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
Bispecific T-cell Engager immobilized on CM5 Chip can bind Human CD3 epsilon, Mouse IgG2a Fc Tag (Cat. No. CDN-H5253) with an affinity constant of 20.1 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
T-cell surface glycoprotein CD3 epsilon chain
- Category：
- Approved Drugs：
4 Details
- Drugs in Clinical Trials：
7 Details
- Highest Development Stage：
Approved
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