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Product Details
Synonyms
CD3 epsilon & CD3 gamma, CD3E & CD3G
Source
Human CD3E&CD3G Heterodimer Protein, Fc Tag&Fc Tag (CDG-H5253) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (CD3E) & Gln 23 - Ser 116 (CD3G) (Accession # NP_000724.1 (CD3E) & AAI13831 (CD3G)).
Predicted N-terminus: Asp 23 (CD3E) & Gln 23 (CD3G)
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
Human CD3E&CD3G Heterodimer Protein, Fc Tag&Fc Tag is produced by co-expression of CD3E and CD3G, has a calculated MW of 38.3 kDa (CD3E) and 36.9 kDa (CD3G). Subunit CD3E is fused with a human IgG1 Fc tag at the C-terminus and subunit CD3G is fused with a human IgG1 Fc tag at the C-terminus. As a result of glycosylation, the protein migrates as 43-55 kDa under reducing (R) condition, and 90-110 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE).
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human CD3E&CD3G Heterodimer Protein, Fc Tag&Fc Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human CD3E&CD3G Heterodimer Protein, Fc Tag&Fc Tag (Cat. No. CDG-H5253) is more than 95% and the molecular weight of this protein is around 80-90 kDa verified by SEC-MALS.
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Bioactivity-ELISA
Immobilized Human CD3E&CD3G Heterodimer Protein, Fc Tag&Fc Tag (Cat. No. CDG-H5253) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3), premium grade (Cat. No. CDE-M120a) with a linear range of 0.8-3 ng/mL (QC tested).
Protocol
Immobilized Human CD3E&CD3G Heterodimer Protein, Fc Tag&Fc Tag (Cat. No. CDG-H5253) at 1 μg/mL (100 μL/well) can bind Mouse Anti Human CD3 with a linear range of 0.8-2 ng/mL (Routinely tested).
Protocol
Immobilized Human CD3E&CD3G Heterodimer Protein, Fc Tag&Fc Tag (Cat. No. CDG-H5253) at 1 μg/mL (100 μL/well) can bind UCHT1 with a linear range of 0.8-3 ng/mL (Routinely tested).
Protocol
Immobilized Human CD3E&CD3G Heterodimer Protein, Fc Tag&Fc Tag (Cat. No. CDG-H5253) at 1 μg/mL (100 μL/well) can bind Bispecific BCMA×CD3 T cell-engaging Antibody with a linear range of 0.8-3 ng/mL (Routinely tested).
Protocol
Customer Reviews Writing Reviews
M***g2023-07-06
LikesThis protein was used to immunize mice for antibody development. Proteins were mixed with CFA/IFA and immunized intraperitoneally. Mice were boosted every 14 days for 5 boosts following initial immunization. Serum titers were measured by ELISA following Days 0, 43, and 65. Some signal was detected in mice by Day 43 however by Day 65 all mice had developed an antibody response.
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