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Product Details
Synonyms
CD47, MER6, IAP, OA3
Source
Biotinylated Cynomolgus / Rhesus macaque CD47 Protein, His,Avitag (CD7-C82E3) is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Glu 141 (Accession # F7A802-1). In the region Gln 19 - Glu 141, the amino acid sequences of Cynomolgus and Rhesus macaque CD47 are identical.
Predicted N-terminus: Gln 19
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 17.5 kDa. The protein migrates as 35-45 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Biotinylated Cynomolgus / Rhesus macaque CD47 Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-ELISA
Immobilized Cynomolgus SIRP alpha, His Tag (Cat. No. SIA-C52H7) at 5 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus / Rhesus macaque CD47 Protein, His,Avitag (Cat. No. CD7-C82E3) with a linear range of 2.5-39 ng/mL (Routinely tested).
Protocol
Immobilized Biotinylated Cynomolgus / Rhesus macaque CD47 Protein, His,Avitag (Cat. No. CD7-C82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Monoclonal Anti-Human CD47 Antibody, Human IgG4 (Cat. No. CD7-M12) with a linear range of 0.2-1.2 ng/mL (QC tested).
Protocol
Immobilized Biotinylated Cynomolgus / Rhesus macaque CD47 Protein, His,Avitag (Cat. No. CD7-C82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Cynomolgus SIRP alpha, Fc Tag (Cat. No. SIA-C5254) with a linear range of 2-39 ng/mL (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Cluster of differentiation 47
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
53 Details
- Highest Development Stage：
Phase 3 Clinical
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