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Détails du produit
Synonyms
CD47, MER6, IAP, OA3
Source
Biotinylated Human CD47, Mouse IgG2a Fc,Avitag (CD7-H82A3) is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Pro 139 (Accession # Q08722-3).
Predicted N-terminus: Gln 19
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a mouse IgG2a Fc tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 42.3 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Affichage des données
SDS-PAGE
Biotinylated Human CD47, Mouse IgG2a Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Biotinylated Human CD47, Mouse IgG2a Fc,Avitag (Cat. No. CD7-H82A3) is more than 95% and the molecular weight of this protein is around 105-115 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human SIRP alpha, Fc Tag (Cat. No. SIA-H5251) at 5 μg/mL (100 μL/well) can bind Biotinylated Human CD47, Mouse IgG2a Fc,Avitag (Cat. No. CD7-H82A3) with a linear range of 5-20 ng/mL (QC tested).
Protocol
Immobilized Biotinylated Human CD47, Mouse IgG2a Fc,Avitag (Cat. No. CD7-H82A3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Monoclonal Anti-Human CD47 Antibody, Human IgG4 with a linear range of 0.2-10 ng/mL (Routinely tested).
Protocol
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G***a2021-08-26
J'aimeThis human CD47-mIgG2aFC worked well in cell based binding assays as determined by flow cytometry. It bound to human SIRP alpha and SIRP gamma expressing cell lines (U87, THP-1, Jurkat T-ALL) as detected by an anti-mouse IgG2a secondary antibody. The binding to SIRP gamma expressing Jurkat cells was reduced when the cells were pre-incubated with SIRP gamma specific antibody clone LSB2.20, as expected.
Introduction de fond
Progrès de frontière
Progrès du développement médicamenteux- Nom anglais：
Cluster of differentiation 47
- Catégorie：
- Nombre de médicaments répertoriés：
0 Détails
- Nombre de médicaments cliniques：
53 Détails
- Étape de développement la plus élevée：
Phase 3 Clinical
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