Détails du produit
Synonyms
CLEC5A, CLECSF5, superfamilymember 5, C-type lectin domain family 5, member A, MDL1, MDL-1, DAP12-associating lectin 1
Source
Human CLEC5A, His Tag (CLA-H5243) is expressed from human 293 cells (HEK293). It contains AA Pro 28 - Lys 188 (Accession # Q9NY25-1).
Predicted N-terminus: His
Molecular Characterization
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 39.0 kDa. The protein migrates as 60-66 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Affichage des données
SDS-PAGE

Human CLEC5A, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS

The purity of Human CLEC5A, His Tag (Cat. No. CLA-H5243) is more than 95% and the molecular weight of this protein is around 70-90 kDa verified by SEC-MALS.
Report
Bioactivity-SPR

Human Galectin-9 immobilized on CM5 Chip can bind Human CLEC5A, His Tag (Cat. No. CLA-H5243) with an affinity constant of 57.9 pM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
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