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Détails du produit
Synonyms
Coagulation Factor III, Tissue Factor, TF, F3, CD142
Source
Human Tissue Factor, His Tag (TF3-H52H5) is expressed from human 293 cells (HEK293). It contains AA Ser 33 - Glu 251 (Accession # P13726-1).
Predicted N-terminus: Ser 33
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 26.7 kDa. The protein migrates as 35-45 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Affichage des données
SDS-PAGE
Human Tissue Factor, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human Tissue Factor, His Tag (Cat. No. TF3-H52H5) is more than 95% and the molecular weight of this protein is around 30-40 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human Tissue Factor, His Tag (Cat. No. TF3-H52H5) at 1 μg/mL (100 μL/well) can bind Anti-TF Antibody, Human IgG1 with a linear range of 0.2-2 ng/mL (Routinely tested).
Protocol
Immobilized Human Tissue Factor, His Tag (Cat. No. TF3-H52H5) at 1 μg/mL (100 μL/well) can bind Tisotumab with a linear range of 0.06-2 ng/mL (Routinely tested).
Protocol
Bioactivity-SPR
Anti-TF mAb immobilized on CM5 Chip via anti-human IgG Fc antibody can bind Human Tissue Factor, His Tag (Cat. No. TF3-H52H5) with an affinity constant of 1.52 nM as determined in SPR assay (Biacore T200) (Routinely tested).
Protocol
Bioactivity
Measured by its ability to activate Coagulation Factor VII in cleaving a fluorogenic peptide substrate Boc-VPR-AMC. The AC50 is <4.0 μg/mL, as measured under the described conditions (QC tested).
Protocol
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Introduction de fond
Progrès de frontière
Progrès du développement médicamenteux- Nom anglais：
Tissue factor
- Catégorie：
- Nombre de médicaments répertoriés：
1 Détails
- Nombre de médicaments cliniques：
8 Détails
- Étape de développement la plus élevée：
Approved
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