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Détails du produit
Synonyms
Coagulation factor Ix, PTC, F9, THPH8, FIX, Plasma Thromboplastic Component, factor Ix
Source
Human Coagulation factor IX Protein, His Tag (FIX-H52H3) is expressed from human 293 cells (HEK293). It contains AA Thr 29 - Thr 461 (Accession # P00740-1).
Predicted N-terminus: Thr 29
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 50.6 kDa. The protein migrates as 60-68 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris,150 mM NaCl,pH8.0 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Affichage des données
SDS-PAGE
Human Coagulation factor IX Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human Coagulation factor IX Protein, His Tag (Cat. No. FIX-H52H3) is more than 90% and the molecular weight of this protein is around 55-70 kDa verified by SEC-MALS.
Report
Bioactivity
Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2. The specific activity is >50 pmol/min/μg (QC tested).
Protocol
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Introduction de fond
Progrès de frontière
Progrès du développement médicamenteux- Nom anglais：
Coagulation factor IX
- Catégorie：
- Nombre de médicaments répertoriés：
33 Détails
- Nombre de médicaments cliniques：
25 Détails
- Étape de développement la plus élevée：
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