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Détails du produit
Synonyms
CTLA4, CD152
Source
Biotinylated Human CTLA-4, His,Avitag (CT4-H82E1) is expressed from human 293 cells (HEK293). It contains AA Ala 37 - Phe 162 (Accession # P16410-1).
Predicted N-terminus: Ala 37
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 17.2 kDa. The protein migrates as 22-30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Affichage des données
SDS-PAGE
Biotinylated Human CTLA-4, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
Bioactivity-ELISA
Immobilized Human B7-1, Fc Tag (Cat. No. B71-H5259) at 2 μg/mL (100 μL/well) can bind Biotinylated Human CTLA-4, His,Avitag (Cat. No. CT4-H82E1) with a linear range of 0.8-25 ng/mL (QC tested).
Protocol
Bioactivity-BLI
Loaded Biotinylated Human CTLA-4, His,Avitag (Cat. No. CT4-H82E1) on SA Biosensor, can bind Human B7-2 Protein, Fc,His Tag (Cat. No. 33031-F001) with an affinity constant of 19.6 nM as determined in BLI assay (ForteBio Octet R8)(Routinely tested).
Protocol
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Introduction de fond
Progrès de frontière
Progrès du développement médicamenteux- Nom anglais：
Cytotoxic T-Lymphocyte-Associated Antigen 4
- Catégorie：
- Nombre de médicaments répertoriés：
5 Détails
- Nombre de médicaments cliniques：
65 Détails
- Étape de développement la plus élevée：
Approved
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