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Rat CTLA-4 / CD152 Protein, Fc Tag (MALS verified)

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Numéro de produit/spécification
Prix
Qté
CT4-R5259-100ug
$260.00
CT4-R5259-1mg
$1815.00
ETA of in-stock products:2 business days
Total des articles Montant du produit$ 0

Détails du produit

  • Synonyms

    CTLA4, CD152

  • Source

    Rat CTLA-4, Fc Tag (CT4-R5259) is expressed from human 293 cells (HEK293). It contains AA Ile 38 - Asp 161 (Accession # Q62859).

    Predicted N-terminus: Ile 38

    Request for sequence
  • Molecular Characterization

    CTLA-4 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 40.2 kDa. The protein migrates as 50-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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  • SDS-PAGE

    CTLA-4 SDS-PAGE

    Rat CTLA-4, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • SEC-MALS

    CTLA-4 SEC-MALS

    The purity of Rat CTLA-4, Fc Tag (Cat. No. CT4-R5259) is more than 95% and the molecular weight of this protein is around 85-110 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     CTLA-4 ELISA

    Immobilized Mouse B7-2, His Tag (Cat. No. CD6-M52H0) at 2 μg/mL (100 μL/well) can bind Rat CTLA-4, Fc Tag (Cat. No. CT4-R5259) with a linear range of 0.2-3 ng/mL (QC tested).

    Protocol

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Introduction de fond

CTLA-4 (Cytotoxic T-Lymphocyte Antigen 4) is also known as CD152 (Cluster of differentiation 152), is a protein receptor that downregulates the immune system. CTLA4 is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. The protein contains an extracellular V domain, a transmembrane domain, and a cytoplasmic tail. Alternate splice variants, encoding different isoforms. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function. Fusion proteins of CTLA4 and antibodies (CTLA4-Ig) have been used in clinical trials for rheumatoid arthritis.

Progrès de frontière

 
Progrès du développement médicamenteux
  • Nom anglais:

    Cytotoxic T-Lymphocyte-Associated Antigen 4

  • Catégorie:

  • Nombre de médicaments répertoriés:

    5 Détails

  • Nombre de médicaments cliniques:

    65 Détails

  • Étape de développement la plus élevée:

    Approved

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