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Product Details
Synonyms
TNFRSF6B, DCR3, TR6, M68
Source
Human DcR3 Protein, His Tag (DC3-H52H3) is expressed from human 293 cells (HEK293). It contains AA Val 30 - Ser 195 (Accession # O95407).
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 20.1 kDa. The protein migrates as 27-33 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
SDS-PAGE
Human DcR3 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human DcR3 Protein, His Tag (Cat. No. DC3-H52H3) is more than 85% and the molecular weight of this protein is around 22-32 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human DcR3 Protein, His Tag (Cat. No. DC3-H52H3) at 5 μg/mL (100 μL/well) can bind Biotinylated Human TL1A Protein, His,Avitag (Cat. No. TLA-H52Q1) with a linear range of 0.2-8 ng/mL (QC tested).
Protocol
Immobilized Human TL1A Protein, Fc Tag at 5 μg/mL (100 μL/well) can bind Human DcR3 Protein, His Tag (Cat. No. DC3-H52H3) with a linear range of 1-31 ng/mL (Routinely tested).
Protocol
Bioactivity-SPR
Human TL1A Protein, Fc Tag (Cat. No. TLA-H5263) captured on Protein A Chip can bind Human DcR3 Protein, His Tag (Cat. No. DC3-H52H3) with an affinity constant of 21.7 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Customer Reviews Writing Reviews
J***h2026-04-03
LikesProducts always arrive very quickly. More quickly then nearly all other competitors, regardless of quantity ordered. We use Acro products as controls that our clients request. They have always been the most reliable in quantity and availability as opposed to BioTechne brands which sometimes take weeks for a quote to be processed, and then another month for delivery. Communication with Acro reps is quicker and more in depth then any of our 200 vendors.
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