Japanese encephalitis virus (strain SA-14) Envelope protein E, His Tag (MALS verified)

Synonyms

Envelope protein E/E protein (JEV)

Source

Japanese encephalitis virus (strain SA-14) Envelope protein E, His Tag (ENE-J52H5) is expressed from human 293 cells (HEK293). It contains AA Phe 295 - Ala 700 (Accession # P27395-1).

Molecular Characterization

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 45.5 kDa. The protein migrates as 48-53 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

Endotoxin

Less than 1.0 EU per μg by the LAL method / rFC method.

Purity

>90% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

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Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

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