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Détails du produit
Synonyms
FcRn, FCGRT & B2M
Source
Biotinylated Cynomolgus / Rhesus macaque FcRn Heterodimer Protein, His,Avitag&Strep II Tag (FCM-C82W5) is expressed from human 293 cells (HEK293). It contains AA Ala 24 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M) (Accession # Q8SPV9-1 (FCGRT) & Q8SPW0-1 (B2M)). In the region Ala 24 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M), the amino acid sequences of Cynomolgus and Rhesus macaque FcRn (FCGRT & B2M) are identical.
Predicted N-terminus: Ala 24 (FCGRT) & Ile 21 (B2M)
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
Biotinylated Cynomolgus / Rhesus macaque FcRn Heterodimer Protein, His,Avitag&Strep II Tag, produced by co-expression of FCGRT and B2M, has a calculated MW of 34.1 kDa (FCGRT) and 13.0 kDa (B2M). Subunit FCGRT is fused with an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag and subunit Beta-2 microglobulin (B2M) is fused with a Strep II tag at the C-terminus. The reducing (R) protein migrates as 40 kDa (FCGRT) and 14 kDa (B2M) respectively due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Affichage des données
SDS-PAGE
Biotinylated Cynomolgus / Rhesus macaque FcRn Heterodimer Protein, His,Avitag&Strep II Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-HPLC
The purity of Biotinylated Cynomolgus / Rhesus macaque FcRn Heterodimer Protein, His,Avitag&Strep II Tag (Cat. No. FCM-C82W5) was greater than 85% as determined by SEC-HPLC.
Bioactivity-SPR
Captured Biotinylated Cynomolgus / Rhesus macaque FcRn Heterodimer Protein, His,Avitag&Strep II Tag (Cat. No. FCM-C82W5) on Biotin CAP - Series S sensor Chip can bind Herceptin® with an affinity constant of 0.241 μM as determined in a SPR assay (Biacore T200) (QC tested).
Protocol
Bioactivity-BLI
Loaded Biotinylated Cynomolgus / Rhesus macaque FcRn Heterodimer Protein, His,Avitag&Strep II Tag (Cat. No. FCM-C82W5) on SA Biosensor, can bind Herceptin with an affinity constant of 0.075 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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Introduction de fond
Progrès de frontière
Progrès du développement médicamenteux- Nom anglais：
IgG receptor FcRn large subunit p51
- Catégorie：
- Nombre de médicaments répertoriés：
4 Détails
- Nombre de médicaments cliniques：
5 Détails
- Étape de développement la plus élevée：
Approved
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