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Détails du produit
Synonyms
FcRn, FCGRT & B2M
Source
MABSol® Biotinylated Mouse FcRn Heterodimer Protein, Avitag,His Tag&Twin-Strep Tag (FCM-M82W6) is expressed from human HEK293 cells. It contains AA Ser 22 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M) (Accession # Q61559-1 (FCGRT) & P01887-1 (B2M)).
Predicted N-terminus: Ser 22 (FCGRT) & Ile 21 (B2M)
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
Biotinylated Mouse FcRn Heterodimer Protein, Avitag,His Tag&Twin-Strep Tag, produced by co-expression of FCGRT and B2M, has a calculated MW of 34.7 kDa (FCGRT) and 14.7 kDa (B2M). Subunit FCGRT is fused with an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag and subunit Beta-2 microglobulin (B2M) is fused with Twin-Strep tag at the C-terminus. The reducing (R) protein migrates as 45-50 kDa (FCGRT) and 15 kDa (B2M) respectively due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Affichage des données
SDS-PAGE
Biotinylated Mouse FcRn Heterodimer Protein, Avitag,His Tag&Twin-Strep Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Biotinylated Mouse FcRn Heterodimer Protein, Avitag,His Tag&Twin-Strep Tag (Cat. No. FCM-M82W6) is more than 95% and the molecular weight of this protein is around 55-65 kDa verified by SEC-MALS.
Report
Bioactivity-BLI
Loaded Biotinylated Mouse FcRn Heterodimer Protein, Avitag,His Tag&Twin-Strep Tag (Cat. No. FCM-M82W6) on SA Biosensor, can bind Herceptin with an affinity constant of 3.38 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
Protocol
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Introduction de fond
Progrès de frontière
Progrès du développement médicamenteux- Nom anglais：
IgG receptor FcRn large subunit p51
- Catégorie：
- Nombre de médicaments répertoriés：
4 Détails
- Nombre de médicaments cliniques：
5 Détails
- Étape de développement la plus élevée：
Approved
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