Détails du produit
Synonyms
FGF-10, Fibroblast growth factor 10, Keratinocyte growth factor 2
Source
Human FGF-10 Protein, His Tag (FG0-H5147) is expressed from E. coli cells. It contains AA Gln 38 - Ser 208 (Accession # O15520-1).
Predicted N-terminus: Met
Request for sequenceMolecular Characterization

Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 21.3 kDa. The protein migrates as 25-27 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Sterility
Negative
Mycoplasma
Negative
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, 0.2 M Arginine, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Affichage des données
SDS-PAGE

Human FGF-10 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-ELISA

Immobilized Human FGF-10 Protein, His Tag (Cat. No. FG0-H5147) at 5 μg/mL (100 μL/well) can bind Human FGF R2 (IIIb), Fc Tag (Cat. No. FGB-H5256) with a linear range of 0.1-6 ng/mL (QC tested).
Protocol
Bioactivity-SPR

Human FGF-10 Protein, His Tag (Cat. No. FG0-H5147) immobilized on CM5 Chip can bind Human FGF R2 (IIIb), Fc Tag (Cat. No. FGB-H5256) with an affinity constant of 4.11 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Application Data

Human FGF-10 Protein, His Tag (Cat. No. FG0-H5147) could support mouse gastric organoid culture after several passages, combined with Human EGF Protein, premium grade (Cat. No. EGF-H5117), Human Noggin Protein, Fc Tag, premium grade (Cat. No. NON-H5257), Human R-Spondin 1 (21-146), His Tag, premium grade (Cat. No. RS6-H4220).
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