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Cynomolgus GDF-15 / MIC-1 Protein, His Tag, low endotoxin

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Numéro de produit/spécification
Prix
Qté
GD5-C5148-100ug
$395.00
GD5-C5148-1mg (500ug X 2)
$2615.00
ETA of in-stock products:2 business days
Total des articles Montant du produit$ 0

Détails du produit

  • Synonyms

    GDF15, GDF-15, MIC-1, MIC1, NAG-1, PDF, PLAB, PTGFB, NRG-1

  • Source

    Cynomolgus GDF-15, His Tag (GD5-C5148) is expressed from E. coli cells. It contains AA Ala 197 - Val 308 (Accession # G7PWZ3-1).

    Predicted N-terminus: Met

    Request for sequence
  • Molecular Characterization

    GDF-15 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 14.4 kDa. The protein migrates as 16 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

  • Endotoxin

    Less than 0.01 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 20 mM Citric acid, pH2.2 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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  • SDS-PAGE

    GDF-15 SDS-PAGE

    Cynomolgus GDF-15, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • Bioactivity-ELISA

     GDF-15 ELISA

    Immobilized Cynomolgus GDF-15, His Tag (Cat. No. GD5-C5148) at 1 μg/mL (100 μL/well) can bind Human GFR alpha-like, Fc Tag (Cat. No. GFE-H5259) with a linear range of 0.8-25 ng/mL (QC tested).

    Protocol

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Introduction de fond

Growth Differentiation Factor 15 (GDF-15), also called Macrophage Inhibitory Cytokine 1 (MIC-1). Expression of MIC-1 mRNA in monocytoid cells is up-regulated by a variety of stimuli associated with activation, including interleukin 1β, tumor necrosis factor α (TNF-α), interleukin 2, and macrophage colony-stimulating factor but not interferon γ, or lipopolysaccharide (LPS). It is highly expressed in cardiomyocytes, adipocytes, macrophages, endothelial cells, and vascular smooth muscle cells in normal and pathological condition. GDF-15 increases during tissue injury and inflammatory states and is associated with cardiometabolic risk. Increased GDF-15 levels are associated with cardiovascular diseases such as hypertrophy, heart failure, atherosclerosis, endothelial dysfunction, obesity, insulin resistance, diabetes, and chronic kidney diseases in diabetes. Increased GDF-15 level is linked with the progression and prognosis of the disease condition.

Progrès de frontière

 
Progrès du développement médicamenteux
  • Nom anglais:

    Growth/differentiation factor 15

  • Catégorie:

  • Nombre de médicaments répertoriés:

    0 Détails

  • Nombre de médicaments cliniques:

    10 Détails

  • Étape de développement la plus élevée:

    Phase 3 Clinical

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