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Détails du produit
Synonyms
GDF-8, Myostatin, GDF8, MSTN, Growth, differentiation factor 8
Source
Human latent GDF-8, His Tag (GD8-H5243) is expressed from human 293 cells (HEK293). It contains AA Asn 24 - Ser 375 (Accession # O14793-1).
Predicted N-terminus: His
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the N-terminus. Pro-GDF8 was predominantly migrated to 45-50 kDa precursor monomers, and latent GDF8 showed 35 kDa prodomain monomer bands together with a 14 kDa growth factor monomer when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
The protein is designed as a dimer.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, 0.2 M Arginine, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Affichage des données
SDS-PAGE
Human latent GDF-8, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human latent GDF-8, His Tag (Cat. No. GD8-H5243) is more than 95% and the molecular weight of this protein is around 80-95 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human latent GDF-8, His Tag (Cat. No. GD8-H5243) at 1 μg/mL (100 μL/well) can bind Biotinylated Human Activin RIIB Protein, Fc,Avitag (Cat. No. ACB-H82F3) with a linear range of 0.2-6 ng/mL (QC tested).
Protocol
Immobilized Human latent GDF-8, His Tag (Cat. No. GD8-H5243) at 1 μg/mL (100 μL/well) can bind Human Follistatin Protein, Fc Tag (Cat. No. FON-H5256) with a linear range of 0.8-25 ng/mL (Routinely tested).
Protocol
Bioactivity-SPR
Human Follistatin Protein, His Tag, premium grade (Cat. No. FON-H52H4) immobilized on CM5 Chip can bind Human latent GDF-8, His Tag (Cat. No. GD8-H5243) with an affinity constant of 364 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Apitegromab captured on Protein A Chip can bind Human latent GDF-8, His Tag (Cat. No. GD8-H5243) with an affinity constant of 9.44 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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Introduction de fond
Progrès de frontière
Progrès du développement médicamenteux- Nom anglais：
Growth/differentiation factor 8
- Catégorie：
- Nombre de médicaments répertoriés：
0 Détails
- Nombre de médicaments cliniques：
8 Détails
- Étape de développement la plus élevée：
NDA/BLA
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