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Product Details
Synonyms
IFN-alpha 1, IFNA1, IFNalpha 1, IFN-alpha-1, LeIF D
Source
Mouse IFN-alpha 1, His Tag (IFA-M52H3) is expressed from human 293 cells (HEK293). It contains AA Cys 24 - Lys 189 (Accession # P01572-1).
Predicted N-terminus: Cys 24
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 21.0 kDa. The protein migrates as 24-27 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Mouse IFN-alpha 1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
Bioactivity-SPR
Mouse IFN-alpha / beta R1, His Tag (Cat. No. IF1-M5225) immobilized on CM5 Chip can bind Mouse IFN-alpha 1, His Tag (Cat. No. IFA-M52H3) with an affinity constant of 2.96 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Mouse IFN-alpha / beta R2, His Tag (Cat. No. IF2-M5225) immobilized on CM5 Chip can bind Mouse IFN-alpha 1, His Tag (Cat. No. IFA-M52H3) with an affinity constant of 0.109 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Mouse IFN-alpha 1, His Tag (Cat. No. IFA-M52H3) on HIS1K Biosensor, can bind Human IFNAR1, Fc Tag (Cat. No. IF1-H5253) with an affinity constant of 0.17 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Mouse IFN-alpha 1, His Tag (Cat. No. IFA-M52H3) on HIS1K Biosensor, can bind Human IFNAR2, Fc Tag (Cat. No. IF2-H5255) with an affinity constant of 1.0 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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