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Détails du produit
Synonyms
IL2RB, RP5-1170K4.6, CD122, P70-75
Source
Human IL-2 R beta Protein, His Tag (CD2-H5221) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Asp 239 (Accession # NP_000869.1).
Predicted N-terminus: Ala 27
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 25.4 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-HPLC.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Affichage des données
SDS-PAGE
Human IL-2 R beta Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-HPLC
The purity of Human IL-2 R beta Protein, His Tag (Cat. No. CD2-H5221) was greater than 90% as determined by SEC-HPLC.
Bioactivity-SPR
Human IL-2 R beta Protein, His Tag (Cat. No. CD2-H5221) captured on CM5 chip via anti-His antibody, can bind Human IL-2, premium grade (Cat. No. IL2-H5215) with an affinity constant of 1.62 μM as determined in a SPR assay (Biacore T200) (QC tested).
Protocol
Human IL-2 R beta Protein, His Tag (Cat. No. CD2-H5221) captured on CM5 chip via anti-His antibody, can bind Human IL-15, premium grade (Cat. No. IL5-H4117) with an affinity constant of 21.7 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Human IL-2 R beta Protein, His Tag (Cat. No. CD2-H5221) on HIS1K Biosensor, can bind Human IL-2, Tag Free with an affinity constant of 0.46 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Bioactivity-CELL BASE
Human IL-2 R beta Protein, His Tag (Cat. No. CD2-H5221) inhibits the IL-15-dependent proliferation of Mo7e cells. The EC50 for this effect is 0.38-0.45 µg/mL (Routinely tested).
Protocol
Avis des utilisateurs Publier un commentaire
V***n2025-02-11
J'aimeI bought this Human recombinant IL-2 R β / CD122 protein to test some anti-CD122 antibodies using ELISA assays where I coat the plate with the CD122 protein. I observed the expected affinity (Kd) results for my antibody (0.3 µg/mL), and I also observed the same results whether I used the CD122 protein freshly reconstituted or freeze-thawed once, showing that this is a really robust purified protein! Lastly, I compared coating my ELISA plates with varying concentrations of CD122 protein (0.25 - 8 µg/mL), finding that I could coat my plates with very little proteins and still observed consistent affinity measurements. This product is a really robust! I will definitely be buying products from Acro Biosystems in the future.
Introduction de fond
CD122 is also known as IL2R beta, is a member of the type I cytokine receptor family. CD122 is the receptor for interleukin-2 and is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2.
Progrès de frontière
Progrès du développement médicamenteux- Nom anglais：
Interleukin-2 receptor beta chain
- Catégorie：
- Nombre de médicaments répertoriés：
0 Détails
- Nombre de médicaments cliniques：
15 Détails
- Étape de développement la plus élevée：
Phase 3 Clinical
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