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Product Details
Laminin 523 (LN523) is a key recombinant basement membrane protein that provides an ideal surface for the adhesion and growth of human pluripotent stem cells while significantly promoting their directed differentiation. Particularly in ophthalmology, it effectively guides the differentiation of stem cells into retinal progenitor cells and supports the formation of retinal organoids containing photoreceptor precursors, while also facilitating the generation and maturation of corneal epithelial cells. It serves as an essential substrate for studying visual development and disease mechanisms.
Expression System
HEK293
Key Parameter
Purity (SDS PAGE)> 90%Mycoplasma TestNegativeSterility TestNegativeEndotoxin Test<0.01 EU per μgHost Cell Protein<0.5 ng/µgHost Cell DNA<0.02 ng/μgFormulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 24 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Affichage des données
Bioactivity-SPR
Human Laminin 523 Protein, premium grade (Cat. No. LA3-H5264) immobilized on CM5 Chip can bind Human ITGA6&ITGB4 Heterodimer Protein, His Tag&Tag Free (Cat. No. IN4-H52W3) with an affinity constant of 137 nM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
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