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Product Details
Synonyms
MSLN, Mesothelin, MPF
Source
FITC-Labeled Human Mesothelin (296-580), His Tag (Cat. No. MSN-HF223) is expressed from human HEK293 cells. It contains AA Glu 296 - Gly 580 (Accession # AAH09272). It is the FITC labeled form of Human Mesothelin (296-580), His Tag.
Predicted N-terminus: Glu 296
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 33 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Conjugate
FITC
Excitation source: 488 nm spectral line, argon-ion laser
Excitation Wavelength: 488 nm
Emission Wavelength: 535 nm
Labeling
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular sieve treatment during purification process.
Protein Ratio
The FITC to protein molar ratio is 0.5-1.5.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
FITC-Labeled Human Mesothelin (296-580), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
Bioactivity-ELISA
Immobilized Anti-Human MSLN MAb at 10 μg/mL (100 μL/well) can bind FITC-Labeled Human Mesothelin (296-580), His Tag (Cat. No. MSN-HF223) with a linear range of 0.078-0.313 μg/mL (Ex.488 nm/Em.535 nm) (QC tested).
Protocol
Evaluation of CAR expression
FACS Analysis of Anti-MSLN CAR Expression
293 cells were transfected with anti-MSLN-scFv and RFP tag. 2e5 of the cells were stained with B. FITC-Labeled Human Mesothelin (296-580), His Tag (Cat. No. MSN-HF223, 1 μg/mL) and C. FITC-labeled protein control. A. Non-transfected 293 cells and C. FITC-labeled protein control were used as negative control. RFP was used to evaluate CAR (anti-MSLN-scFv) expression and FITC was used to evaluate the binding activity of FITC-Labeled Human Mesothelin (296-580), His Tag (Cat. No. MSN-HF223).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Mesothelin
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
55 Details
- Highest Development Stage：
Phase 2 Clinical
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