FITC-Labeled Human MSP1D1 Protein, His Tag (Nanodisc)

Application

1. Cell based assay (FACS or functional assay)
2. Affinity testing (SPR / BLI)
3. Screening (ELISA)

Synonyms

MSP1D1

Source

Nanodisc FITC-Labeled Human MSP1D1 Protein, His Tag is expressed from E. coli system. It contains AA Gly 1 - Gln 211. It is the FITC labeled form of Human MSP1D1 Protein, His Tag.

Predicted N-terminus: Met

Molecular Characterization

This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 24.7 kDa. The protein migrates as 20-30 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE). This protein is used together with nanodisc protein as isotype control.

1. Structure

   

   Nanodisc membrane protein is wrapped by a synthetic phospholipid bilayer membrane structure, composed of membrane scaffold proteins (MSPs) and phospholipids.

2. Synthesis Process

   

   Nanodisc reconstitution is a self-assembly process that starts from purified, detergent-solubilized membrane proteins and reconstitutes them into synthetic phospholipid bilayers stabilized by MSP1D1 membrane scaffold protein. This creates a stable, detergent-free environment that preserves native conformation and biological activity, with excellent homogeneity and full compatibility with cell-based assays such as FACS and binding assays in antibody discovery and screening (ELISA),etc.

Conjugate

FITC

Excitation source: 488 nm spectral line, argon-ion laser

Excitation Wavelength: 488 nm

Emission Wavelength: 535 nm

Labeling

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular sieve treatment during purification process.

Purity

>90% as determined by SDS-PAGE.

Formulation

Supplied as 0.2 μm filtered solution in 20 mM HEPES, 150 mM NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

Shipping and Storage

This product is supplied and shipped on dry ice.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. The product MUST be stored at -70°C or lower upon receipt;
2. -70°C for 12 months under sterile conditions.

ACRO Quality Management System

1. QMS(ISO, GMP)
2. Quality Advantages
3. Quality Control Process