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Product Details
Synonyms
CD56, MSK39, NCAM1, N-CAM-1
Source
Alexa Fluor 488-Labeled Human NCAM-1 Protein, His Tag (NC1-HA2H3) is produced via conjugation of AF488 to Human NCAM-1, His Tag with a new generation site-specific technology under Star Staining labeling platform. Human NCAM-1, His Tag is expressed from human 293 cells (HEK293). It contains AA Leu 20 - Gly 718 (Accession # P13591-2).
Predicted N-terminus: Leu 20
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 94.0 kDa. The protein migrates as 100-130 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Conjugate
AF488
Excitation Wavelength: 488 nm
Emission Wavelength: 517 nm
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-HPLC.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Alexa Fluor 488-Labeled Human NCAM-1 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-HPLC
The purity of Alexa Fluor 488-Labeled Human NCAM-1 Protein, His Tag (Cat. No. NC1-HA2H3) was greater than 90% as determined by SEC-HPLC.
Bioactivity-ELISA
Immobilized Alexa Fluor 488-Labeled Human NCAM-1 Protein, His Tag (Cat. No. NC1-HA2H3) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-NCAM-1 (High) Antibody, Human IgG1 with a linear range of 0.002-0.125 μg/mL (QC tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Neural cell adhesion molecule 1
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
5 Details
- Highest Development Stage：
Phase 2 Clinical
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