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Human NKG2A&CD94 Protein, His Tag, low endotoxin

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Numéro de produit/spécification
Prix
Qté
NC4-H52H3-100ug
$395.00
NC4-H52H3-1mg (500ug X 2)
$2615.00
ETA of in-stock products:2 business days
Total des articles Montant du produit$ 0

Détails du produit

  • Synonyms

    NKG2A & CD94

  • Source

    Human NKG2A&CD94 Protein, His Tag (NC4-H52H3) is expressed from human 293 cells (HEK293). It contains AA Ala 113 - Leu 233 (NKG2A) & Asp 57 - Ile 179 (CD94) (Accession # P26715-1 (NKG2A) & Q13241-1 (CD94)).

  • Molecular Characterization

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 30.7 kDa. The protein migrates as 40-55 kDa and 65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 0.01 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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  • SDS-PAGE

    NKG2A & CD94 SDS-PAGE

    Human NKG2A&CD94 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • Bioactivity-ELISA

     NKG2A & CD94 ELISA

    Immobilized Human NKG2A&CD94 Protein, His Tag (Cat. No. NC4-H52H3) at 2 μg/mL (100 μL/well) can bind Anti-NKG2A Antibody, Human IgG1 with a linear range of 0.1-2 ng/mL (QC tested).

    Protocol

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Introduction de fond

CD94 plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells. KLRD1 (CD94) is an antigen preferentially expressed on NK cells and is classified as a type II membrane protein because it has an external C terminus. NKG2A/CD159a is a transmembrane protein belonging to the CD94/NKG2 family of C-type lectin-like receptors that inhibits innate immune system activation. CD94 pairs with the NKG2 molecule as a heterodimer. The CD94/NKG2 complex, on the surface of natural killer cells interacts with Human Leukocyte Antigen (HLA)-E on target cells.

Progrès de frontière

 
Progrès du développement médicamenteux
  • Nom anglais:

    NK cell lectin-like receptor subfamily C member 1

  • Catégorie:

  • Nombre de médicaments répertoriés:

    0 Détails

  • Nombre de médicaments cliniques:

    6 Détails

  • Étape de développement la plus élevée:

    Phase 3 Clinical

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