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Human NKp30 / NCR3 / CD337 Protein, Llama IgG2b Fc Tag, low endotoxin

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Numéro de produit/spécification
Prix
Qté
NC3-H5256-100ug
$390.00
NC3-H5256-1mg (500ug X 2)
$2575.00
ETA of in-stock products:2 business days
Total des articles Montant du produit$ 0

Détails du produit

  • Synonyms

    NCR3, CD337, NKp30, 1C7, LY117, MALS

  • Source

    Human NKp30, Llama IgG2b Fc Tag (NC3-H5256) is expressed from human 293 cells (HEK293). It contains AA Leu 19 - Gly 135 (Accession # NP_667341.1).

    Predicted N-terminus: Leu 19

    Request for sequence
  • Molecular Characterization

    NKp30 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a llama IgG2b Fc tag at the C-terminus.

    The protein has a calculated MW of 40.7 kDa. The protein migrates as 50-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 0.01 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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  • SDS-PAGE

    NKp30 SDS-PAGE

    Human NKp30, Llama IgG2b Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • Bioactivity-ELISA

     NKp30 ELISA

    Immobilized Human NKp30, Llama IgG2b Fc Tag (Cat. No. NC3-H5256) at 5 μg/mL (100 μL/well) can bind Biotinylated Human B7-H6, Fc,Avitag (Cat. No. B76-H82Wb) with a linear range of 0.6-20 ng/mL (QC tested).

    Protocol
  •  NKp30 ELISA

    Immobilized Human B7-H6, His Tag (Cat. No. B76-H52H8) at 5 μg/mL (100 μL/well) can bind Human NKp30, Llama IgG2b Fc Tag (Cat. No. NC3-H5256) with a linear range of 0.6-78 ng/mL (Routinely tested).

    Protocol

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Introduction de fond

Natural cytotoxicity triggering receptor 3 (NCR3) is also known as Activating natural killer receptor p30, Natural killer cell p30-related protein (NK-p30), CD antigen CD337, 1C7, LY117. NCR3 /CD337 /NKp30 belongs to the natural cytotoxicity receptor (NCR) family. NCR3 /NKp30 contains one Ig-like (immunoglobulin-like) domain. NCR3 /NKp30 is selectively expressed by all resting and activated NK cells and weakly expressed in spleen. NCR3 is homodimer in the unliganted form. NCR3 interacts with CD3Z. NCR3 interacts with and is activated by binding to NCR3LG1 or BAG6. Engagement of NCR3 by BAG6 also promotes dendritic cell (DC) maturation, both through killing those DCs that did not properly acquire a mature phenotype, and inducing NK cells to release TNFA and IFNG, which promotes DC maturation.

Progrès de frontière

 
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