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Détails du produit
Synonyms
Natural cytotoxicity triggering receptor 2, NKp44, NCR2, CD336, LY95
Source
Human NKp44, Llama IgG2b Fc Tag (NC2-H5258) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Ala 192 (Accession # O95944-1).
Predicted N-terminus: Gln 22
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a llama IgG2b Fc tag at the C-terminus.
The protein has a calculated MW of 46.5 kDa. The protein migrates as 55-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Affichage des données
SDS-PAGE
Human NKp44, Llama IgG2b Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-ELISA
Immobilized PDGF-DD Protein, Human at 5 μg/mL (100 μL/well) can bind Human NKp44, Llama IgG2b Fc Tag (Cat. No. NC2-H5258) with a linear range of 0.008-0.25 μg/mL (Routinely tested).
Protocol
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Natural cytotoxicity triggering receptor 2
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