Détails du produit
Synonyms
neurotrophic factor 4, neurotrophic factor 5, neurotrophin 4, neurotrophin 5, neurotrophin-5, NT4, NT-4, 5, NT-5, NTF4, NTF5, GLC10
Source
Human NT-4, Tag Free (NT4-H5114) is expressed from E. coli cells. It contains AA Gly 81 - Ala 210 (Accession # P34130-1).
Predicted N-terminus: Met
Request for sequenceMolecular Characterization

Other Tags and Version Biotin & Other Labeled Version
This protein carries no "tag".
The protein has a calculated MW of 14.1 kDa. The protein migrates as 15 kDa under reducing (R) condition (SDS-PAGE).
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Affichage des données
SDS-PAGE

Human NT-4, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS

The purity of Human NT-4, Tag Free (Cat. No. NT4-H5114) is more than 95% and the molecular weight of this protein is around 28-40 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA

Immobilized Human NT-4, Tag Free (Cat. No. NT4-H5114) at 5 μg/mL (100 μL/well) can bind Human TrkB, Fc Tag (Cat. No. NT2-H5254) with a linear range of 2-10 ng/mL (QC tested).
Protocol
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