Collectionner le produit
Détails du produit
Synonyms
PDCD1, PD1, CD279, SLEB2
Source
Human PD-1 Full Length, His Tag (PD1-H52H6) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Leu 288 (Accession # Q15116-1).
Predicted N-terminus: Leu 25
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 30.7 kDa. The protein migrates as 40-120 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>80% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM NaCl, pH8.0 with detergent with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
Les clients ont également consulté
Affichage des données
SDS-PAGE
Human PD-1 Full Length, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 80% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-ELISA
Immobilized Human PD-1 Full Length, His Tag (Cat. No. PD1-H52H6) at 0.2 μg/mL (100 μL/well) can bind Nivolumab with a linear range of 0.4-3 ng/mL (QC tested).
Protocol
Immobilized Human PD-1 Full Length, His Tag (Cat. No. PD1-H52H6) at 0.5 μg/mL (100 μL/well) can bind Biotinylated Human PD-L1, Fc,Avitag (Cat. No. PDL-H82F2) with a linear range of 0.001-0.125 μg/mL (QC tested).
Protocol
Immobilized Human PD-1 Full Length, His Tag (Cat. No. PD1-H52H6) at 0.2 μg/mL (100 μL/well) can bind Human PD-L1, Mouse IgG1 Fc Tag, low endotoxin (Cat. No. PD1-H52A3) with a linear range of 0.01-0.313 μg/mL (Routinely tested).
Protocol
Immobilized Human PD-1 Full Length, His Tag (Cat. No. PD1-H52H6) at 0.2 μg/mL (100 μL/well) can bind Human PD-L2, Mouse IgG1 Fc Tag (Cat. No. PD2-H52A5) with a linear range of 0.5-8 ng/mL (Routinely tested).
Protocol
Avis des utilisateurs Publier un commentaire
Introduction de fond
Progrès de frontière
Progrès du développement médicamenteux- Nom anglais：
Programmed cell death protein 1
- Catégorie：
- Nombre de médicaments répertoriés：
22 Détails
- Nombre de médicaments cliniques：
193 Détails
- Étape de développement la plus élevée：
Approved
Contact Us
Drug Candidate Licensing
