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Product Details
Synonyms
TNFRSF1B, CD120b, TBPII, TNF-R-II, TNF-R75, TNFBR, TNFR1B, TNFR2, TNFR80, p75, p75TNFR
Source
Mouse & Human TNFR2 Protein, His Tag (TN2-M52H3) is expressed from human 293 cells (HEK293). It contains AA Val 23 - Ala 120 (Mouse TNFR2) & Cys 120 - Thr 201(Human TNFR2) (Accession # P25119 & P20333-1).
Predicted N-terminus: Val 23
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 21.6 kDa. The protein migrates as 30-33 kDa and 35-37 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Mouse & Human TNFR2 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Mouse & Human TNFR2 Protein, His Tag (Cat. No. TN2-M52H3) is more than 95% and the molecular weight of this protein is around 22-32 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Mouse TNF-alpha Protein, Fc Tag (Cat. No. TNA-M5257) at 5 μg/mL (100 μL/well) can bind Mouse / Human TNFR2 Protein, His Tag (Cat. No. TN2-M52H3) with a linear range of 0.01-1.25 μg/mL (QC tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Tumor necrosis factor receptor superfamily member 1B
- Category：
- Approved Drugs：
1 Details
- Drugs in Clinical Trials：
8 Details
- Highest Development Stage：
Approved
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