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Détails du produit
Synonyms
RANKL, CD254, TRANCE, OPGL, ODF
Source
Human TNFSF11 Protein, His Tag (RAL-H5240) is expressed from human 293 cells (HEK293). It contains AA Gly 64 - Asp 245 (Accession # AAC51762.1).
Predicted N-terminus: His
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 22.4 kDa. The protein migrates as 28-35 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a trimer.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Affichage des données
SDS-PAGE
Human TNFSF11 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human TNFSF11 Protein, His Tag (Cat. No. RAL-H5240) is more than 95% and the molecular weight of this protein is around 75-110 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human TNFSF11 Protein, His Tag (Cat. No. RAL-H5240) at 2 μg/mL (100 μL/well) can bind Human RANK Protein, Mouse IgG2a Fc Tag (Cat. No. RAK-H5251) with a linear range of 0.2-5 ng/mL (QC tested).
Protocol
Immobilized Human OPG, Fc Tag (Cat. No. TNB-H5259) at 2 μg/mL (100 μL/well) can bind Human TNFSF11 Protein, His Tag (Cat. No. RAL-H5240) with a linear range of 0.2-2 ng/mL (Routinely tested).
Protocol
Bioactivity-SPR
Anti-TNFSF11 (human IgG1) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human TNFSF11 Protein, His Tag (Cat. No. RAL-H5240) with an affinity constant of 0.274 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
Captured Human RANK Protein, Mouse IgG2a Fc Tag (Cat. No. RAK-H5251) on CM5 chip via Anti-Mouse antibodies surface can bind Human TNFSF11 Protein, His Tag (Cat. No. RAL-H5240) with an affinity constant of 0.309 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Human RANK Protein, Mouse IgG2a Fc Tag (Cat. No. RAK-H5251) on AMC Biosensor, can bind Human TNFSF11 Protein, His Tag (Cat. No. RAL-H5240) with an affinity constant of 0.409 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Anti-TNFSF11 MAb (human IgG1) on AHC Biosensor, can bind Human TNFSF11 Protein, His Tag (Cat. No. RAL-H5240) with an affinity constant of 0.239 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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Introduction de fond
Progrès de frontière
Progrès du développement médicamenteux- Nom anglais：
Tumor necrosis factor ligand superfamily member 11
- Catégorie：
- Nombre de médicaments répertoriés：
18 Détails
- Nombre de médicaments cliniques：
18 Détails
- Étape de développement la plus élevée：
Approved
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