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Human TRAIL R2 / DR5 / TNFRSF10B Protein, His Tag, low endotoxin

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Numéro de produit/spécification
Prix
Qté
TR2-H5229-100ug
$285.00
TR2-H5229-1mg (500ug X 2)
$1815.00
ETA of in-stock products:2 business days
Total des articles Montant du produit$ 0

Détails du produit

  • Synonyms

    TNFRSF10B, TRAILR2, TRAIL-R2, CD262, DR5, KILLER, TRICK2, ZTNFR9, TRICKB

  • Source

    Human TRAIL R2, His Tag (TR2-H5229) is expressed from human 293 cells (HEK293). It contains AA Ile 56 - Glu 182 (Accession # NP_003833).

    Predicted N-terminus: Ile 56

    Request for sequence
  • Molecular Characterization

    TRAIL R2 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 15.4 kDa. The protein migrates as 16-18 kDa, 19-20 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 0.01 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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  • SDS-PAGE

    TRAIL R2 SDS-PAGE

    Human TRAIL R2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • Bioactivity-ELISA

     TRAIL R2 ELISA

    Immobilized Human TRAIL R2, His Tag (Cat. No. TR2-H5229) at 5 μg/mL (100 μL/well) can bind Human TRAIL, Mouse IgG2a Fc Tag with a linear range of 2-63 ng/mL (QC tested).

    Protocol

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Introduction de fond

Tumor necrosis factor receptor superfamily member 10B (TNFRSF10B) is also known as TNF-related apoptosis-inducing ligand receptor 2 (TRAILR2), Death receptor 5 (DR5), CD262, KILLER, is a member of the TNF-receptor superfamily, and contains an intracellular death domain. TNFRSF10B / DR-5 is widely expressed in adult and fetal tissues; very highly expressed in tumor cell lines. TRAILR2 / CD262 / DR5 is the receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD (a death domain containing adaptor protein) of TRAIL-R2 / TNFRSF10B recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. CD262 / DR5 Promotes the activation of NF-kappa-B. DR5 is essential for ER stress-induced apoptosis and is regulated by p53/TP53.

Progrès de frontière

 
Progrès du développement médicamenteux
  • Nom anglais:

    Tumor necrosis factor receptor superfamily member 10B

  • Catégorie:

  • Nombre de médicaments répertoriés:

    1 Détails

  • Nombre de médicaments cliniques:

    10 Détails

  • Étape de développement la plus élevée:

    Approved

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