Collectionner le produit
Détails du produit
Synonyms
TACSTD2, GA733-1, M1S1, TROP2
Source
Human TROP-2, His Tag (TR2-H5223) is expressed from human 293 cells (HEK293). It contains AA Gln 31 - Thr 274 (Accession # P09758-1).
Predicted N-terminus: Gln 31
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 29.4 kDa. The protein migrates as 43-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution.
ACRO Quality Management System
Les clients ont également consulté
Affichage des données
SDS-PAGE
Human TROP-2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human TROP-2, His Tag (Cat. No. TR2-H5223) is more than 95% and the molecular weight of this protein is around 33-50 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human TROP-2, His Tag (Cat. No. TR2-H5223) at 1 μg/mL (100 μL/well) can bind Mouse Monoclonal Antibody Against Human TROP-2, Mouse IgG1 with a linear range of 0.1-2 ng/mL (QC tested).
Protocol
Bioactivity-TR-FRET
In this TR-FRET assay, Monoclonal Anti-His Tag Antibody Europium chelate (for TR-FRET) (Cat. No. FRT-TD05) is used as the Donor and Monoclonal Anti-Human IgG Antibody FA-Labeled (for TR-FRET) (Cat. No. FRT-TA02) is used as the Acceptor. Human TROP-2, His Tag (Cat. No. TR2-H5223), at 1.25 ng/well, can bind to the Datopotamab with a linear detection range of 8-0.001 μg/mL. (Routinely tested)
Bioactivity-SPR
Captured Trop2 antibody on CM5 chip via anti-mouse antibodies surface can bind Human TROP-2, His Tag (Cat. No. TR2-H5223) with an affinity constant of 5.98 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
Avis des utilisateurs Publier un commentaire
V***w2023-02-21
J'aimeAcro and their customer support are super helpful. Products ordered arrived in a timely manner. Customer support personal are as friendly as they are insightful. Never have they disappointed when purchasing any of their products. Such a user friendly website and if anything is not found customer support never backs down to help out! Acro’s personal are very friendly and knowledgeable. They were friendly and helpful when resolving this issue.- E***l2022-10-26 J'aimeAcro’s customer support is always super helpful and quick to respond. When products are ordered, the arrive in a timely manner. Acro’s personal are very friendly and knowledgeable. They never disappoint when it comes to finding a solution to a problem. Having such a user-friendly website helps a lot in finding the right product needed and if something cannot be found customer support always finds a way!
1***32022-08-10 J'aimeACRO's antigens are well solubilized and encapsulated for antibody screening, and the method is now being established smoothly. Also, they provide us with complete technical support which can be given in various aspects for any problems that arise during the test.
Introduction de fond
Progrès de frontière
Progrès du développement médicamenteux- Nom anglais：
Tumor-associated calcium signal transducer 2
- Catégorie：
- Nombre de médicaments répertoriés：
3 Détails
- Nombre de médicaments cliniques：
47 Détails
- Étape de développement la plus élevée：
Approved
Contact Us
Drug Candidate Licensing
