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Human TSPAN8 Protein, His Tag, low endotoxin

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Numéro de produit/spécification
Prix
Qté
TS8-H52H3-100ug
$360.00
TS8-H52H3-1mg
$2615.00
ETA of in-stock products:2 business days
Total des articles Montant du produit$ 0

Détails du produit

  • Synonyms

    CO-029, tetraspanin 8, TM4SF3, TM4SF3tspan-8, Transmembrane 4 superfamily member 3tetraspanin-8, TSPAN8, Tspan-8, Tumor-associated antigen CO-029

  • Source

    Human TSPAN8, His Tag (TS8-H52H3) is expressed from human 293 cells (HEK293). It contains AA Lys 110 - Asn 205 (Accession # P19075).

    Predicted N-terminus: His

    Request for sequence
  • Molecular Characterization

    TSPAN8 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 12.9 kDa. The protein migrates as 14 kDa and 17-19 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 0.01 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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  • SDS-PAGE

    TSPAN8 SDS-PAGE

    Human TSPAN8, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    TSPAN8 SEC-MALS

    The purity of Human TSPAN8, His Tag (Cat. No. TS8-H52H3) is more than 95% and the molecular weight of this protein is around 13-20 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     TSPAN8 ELISA

    Immobilized Human TSPAN8, His Tag (Cat. No. TS8-H52H3) at 1 μg/mL (100 μL/well) can bind Anti-TSPAN8 mIgG2b Antibody with a linear range of 0.06-2 ng/mL (QC tested).

    Protocol
  •  TSPAN8 ELISA

    Immobilized Anti-TSPAN8 mIgG2b Antibody at 1 μg/mL (100 μL/well) can bind Human TSPAN8, His Tag (Cat. No. TS8-H52H3) with a linear range of 4-63 ng/mL (Routinely tested).

    Protocol

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Introduction de fond

Tspan8 is 1 of the 33 mammalian members of the tetraspanin family, composed of transmembrane proteins that organize laterally, together or with other membrane partners such as integrins, to form ‘tetraspanin webs’. These platforms signal within cells to regulate many cellular processes: adhesion, migration, invasion or survival Tspan8 has been implicated in many types of cancer.Overexpression was reported in glioma and colorectal, esophageal, hepatic, gastric and pancreatic carcinoma. Tspan8 exerts a pro-invasive function by controlling cell–cell and cell–matrix interactions through its association with membrane partners such as α6β4 integrin-protein kinase C (PKC)-activated, E-cadherin, EpCAM, claudin-7 and CD44. Moreover, Tspan8 may be a promising new therapeutic target, as Tspan8-specific antibodies were shown to reduce cell motility, block tumor angiogenesis in vivo.

Progrès de frontière

 
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