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Détails du produit
Synonyms
VCAM1, CD106, INCAM-100, V-CAM 1, VCAM-1
Source
Human VCAM-1, His Tag (VC1-H5224) is expressed from human 293 cells (HEK293). It contains AA Phe 25 - Pro 697 (Accession # NP_001069).
Predicted N-terminus: Phe 25
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 74.9 kDa. The protein migrates as 85-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Affichage des données
SDS-PAGE
Human VCAM-1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Human VCAM-1, His Tag (Cat. No. VC1-H5224) is more than 95% and the molecular weight of this protein is around 78-106 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human VCAM-1, His Tag (Cat. No. VC1-H5224) at 5 μg/mL (100 μL/well) can bind Biotinylated Human ITGA4&ITGB1 Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT1-H82W1) with a linear range of 4-78 ng/mL (QC tested).
Protocol
Bioactivity-SPR
Human VCAM-1, His Tag (Cat. No. VC1-H5224) immobilized on CM5 Chip can bind Human ITGA4&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W1) with an affinity constant of 51.3 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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Introduction de fond
Progrès de frontière
Progrès du développement médicamenteux- Nom anglais：
Vascular cell adhesion molecule 1
- Catégorie：
- Nombre de médicaments répertoriés：
2 Détails
- Nombre de médicaments cliniques：
1 Détails
- Étape de développement la plus élevée：
Approved
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