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Détails du produit
Synonyms
RP1-261G23.1, MGC70609, MVCD1, VEGFA, VPF
Source
Human VEGF165, His Tag (VE5-H5248) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 191 (Accession # P15692-4). In the region Ala 27 - Arg 191, the amino acid sequences of VEGF165 from Human, Cynomolgus and Rhesus macaque are identical.
Predicted N-terminus: His
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 21.1 kDa. The protein migrates as 27-28 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a dimer.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Affichage des données
SDS-PAGE
Human VEGF165, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human VEGF165, His Tag (Cat. No. VE5-H5248) is more than 95% and the molecular weight of this protein is around 42-60 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human VEGF R1&VEGF R2, Fc Tag at 2 μg/mL (100 μL/well) can bind Human VEGF165, His Tag (Cat. No. VE5-H5248) with a linear range of 0.004-0.063 μg/mL (QC tested).
Protocol
Bioactivity-TR-FRET
In this TR-FRET assay, Streptavidin Protein Europium chelate (for TR-FRET) (Cat. No. FRT-TD09) is used as the Donor and Monoclonal Anti-His Tag Antibody, Rabbit IgG FA-Labeled (for TR-FRET) (Cat. No. FRT-TA11) is used as the Acceptor. Human VEGF165, His Tag (Cat. No. VE5-H5248), at 1.25 ng/well and Biotinylated Human PD-1, Fc,Avitag (Cat. No. PD1-H82F1), at 1.25 ng/well, can bind to the Ivonescimab with a linear detection range of 2-0.002 μg/mL (Routinely tested).
Bridging ELISA
Validated with Bispecific Antibody.
Immobilized Human VEGF165 Protein, His Tag, low endotoxin (MALS verified) (Cat. No. VE5-H5248) at 1 μg/mL, add increasing concentrations of Ivonescimab and then add Biotinylated Cynomolgus PD-1 / PDCD1 Protein, Fc,Avitag™ (MALS verified) (Cat. No. PD1-C82F7) at 0.2 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 0.244 ng/mL (Routinely tested).
Validated with Bispecific Antibody.
Immobilized Human VEGF165, His Tag (Cat. No. VE5-H5248) at 1 μg/mL, add increasing concentrations of Pumitamig and then add Biotinylated Human PD-L1, Avitag,His Tag (Cat. No. PD1-H82E5) at 0.2 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 0.31 ng/mL (Routinely tested).
Bioactivity-SPR
Avastin (Bevacizumab) captured on CM5 chip via ant-human IgG Fc antibodies surface, can bind Human VEGF165 Protein, His Tag (Cat. No. VE5-H5248) with an affinity constant of 1.03 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Human NRP1, Fc Tag (Cat. No. NR1-H5252) on Protein A Biosensor, can bind Human VEGF165, His Tag (Cat. No. VE5-H5248) with an affinity constant of 24.2 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Biotinylated Human VEGF R1, His,Avitag (Cat. No. VE1-H82E3) on SA Biosensor, can bind Human VEGF165, His Tag (Cat. No. VE5-H5248) with an affinity constant of 0.236 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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Introduction de fond
Progrès de frontière
Progrès du développement médicamenteux- Nom anglais：
Vascular endothelial growth factor A
- Catégorie：
- Nombre de médicaments répertoriés：
54 Détails
- Nombre de médicaments cliniques：
61 Détails
- Étape de développement la plus élevée：
Approved
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