Product Details
Source
Biotinylated Anti-Bevacizumab Antibody (AY13) is a mouse monoclonal antibody derived from hybridoma cells fused with SP2/0 myeloma cells and murine B lymphocytes.
Antibody Type
Hybridoma Monoclonal
Clone
AY13
Isotype
Mouse IgG1, Kappa
Host Species
Mouse
Immunogen
Bevacizumab
Conjugate
Biotin
Specificity
Recognizes Bevacizumab specifically.
Application
ApplicationRecommended UsageELISA1:100-1:1000Purification
Protein A purified / Protein G purified.
Aggregation
Less than 5%, as determined by SEC-MALS.
Concentration
Please refer to the Certificate of Analysis (CoA).
Form
Lyophilized
Formulation
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4) containing 0.03% PF68, with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
Shipping
Lyophilized product is shipped at ambient temperature.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution.
Notices
Product Specific Notices: For research use only.
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Performance Data
Bioactivity-ELISA

Detection of bevacizumab by bridging ELISA in serum. Immobilized Anti-Bevacizumab Antibody (AY10) (Cat. No. BEB-Y10) at 2 μg/mL, add increasing concentrations of bevacizumab (10% human serum) and then add Biotinylated Anti-Bevacizumab Antibody (AY13) (Cat. No. BEB-BY13) at 2 μg/mL. Detection was performed using HRP-conjugated streptavidin with a sensitivity of 0.4 μg/mL (QC tested).
Protocol
Immobilized bevacizumab at 1 μg/mL (100 μL/well) can bind Biotinylated Anti-Bevacizumab Antibody (AY13) (Cat. No. BEB-BY13) with a linear range of 0.78-25.5 ng/mL.
Protocol
Serial dilutions of Biotinylated Anti-Bevacizumab Antibody (AY13) (Cat. No. BEB-BY13) were added into Human VEGF165, premium grade (Cat. No. VE5-H4210): Human Anti-VEGFA(AVASTIN), Human IgG1 (D239E, L241M) | Human Kappa binding reactions. The half maximal inhibitory concentration (IC50) is 1.032 μg/mL (Routinely tested).
Protocol
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