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Human APP / Abeta42 Protein, His Tag

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Numéro de produit/spécification
Prix
Qté
APP-H51H6-100ug
$380.00
APP-H51H6-500ug
$1150.00
ETA of in-stock products:2 business days
Total des articles Montant du produit$ 0

Détails du produit

  • Synonyms

    ABPP, APPI, Amyloid-beta A4 protein

  • Source

    Human Abeta42, His Tag (APP-H51H6) is expressed from E. coli cells. It contains AA Asp 672 - Ala 713 (Accession # P05067-1).

    Predicted N-terminus: Met

    Request for sequence
  • Molecular Characterization

    APP Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 6.3 kDa. The protein migrates as 8-9 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 20 mM NaOH with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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  • SDS-PAGE

    APP SDS-PAGE

    Human Abeta42, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • Bioactivity-ELISA

     APP ELISA

    Immobilized Human Abeta42, His Tag (Cat. No. APP-H51H6) at 1 μg/mL (100 μL/well) can bind Biosimilar Aducanumab with a linear range of 0.05-2 ng/mL (QC tested).

    Protocol

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Introduction de fond

Amyloid precursor protein (APP) is a type I integral membrane protein ubiquitously expressed in many tissues and concentrated in the synapses of neurons. It has three predominant splice variants: APP695, APP751, and APP770. The majority of APP is cleaved at the plasma membrane by the α-secretase in the non-amyloidogenic pathway. The amyloidogenic pathway starts with β-secretase cleavage by BACE1 on the N-terminal part of the Aβ domain, releasing sAPPβ from a membrane-anchored fragment named βCTF or C99, which is subsequently cleaved by γ-secretase to release Aβ.

Progrès de frontière

 
Progrès du développement médicamenteux
  • Nom anglais:

    Beta amyloid A4 protein

  • Catégorie:

  • Nombre de médicaments répertoriés:

    13 Détails

  • Nombre de médicaments cliniques:

    54 Détails

  • Étape de développement la plus élevée:

    Approved

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