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Product Details
Synonyms
CD39, ENTPD1, NTPDase 1, Entpd1, Ecto-ATPDase 1, Ecto-ATPase 1
Source
Human CD39 (Q96A, N99A, E143A, R147E) Protein, His Tag (CD9-H52H5) is expressed from human 293 cells (HEK293). It contains AA Thr 38 - Val 478 (Accession # P49961-1 (Q96A, N99A, E143A, R147E)).
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 52.1 kDa. The protein migrates as 60-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Supplied as 0.2 μm filtered solution in 20 mM Tris,150 mM NaCl,pH8.0 with trehalose as protectant.
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Shipping
This product is supplied and shipped with dry ice, please inquire the shipping cost.
Storage
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
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Performance Data
SDS-PAGE
Human CD39 (Q96A, N99A, E143A, R147E) Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Human CD39 (Q96A, N99A, E143A, R147E) Protein, His Tag (Cat. No. CD9-H52H5) is more than 90% and the molecular weight of this protein is around 65-85 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human CD39 / ENTPD1 (Q96A, N99A, E143A, R147E), His Tag (Cat. No. CD9-H52H5) at 1 μg/mL (100 μL/well) can bind X-IgG1 with a linear range of 0.5-3 ng/mL (Routinely tested).
Protocol
Bioactivity
Measured by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-triphosphate (ATP). The specific activity is > 8,000 pmol/min/μg (QC tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Ectonucleoside triphosphate diphosphohydrolase 1
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
11 Details
- Highest Development Stage：
Phase 2 Clinical
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