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APC-Labeled Human CD3E & CD3D Protein, His Tag&Tag FreeStar Staining

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Numéro de produit/spécification
Prix
Qté
CDD-HA2H3-25ug
$420.00
CDD-HA2H3-200ug
$2670.00
ETA of in-stock products:2 business days
Total des articles Montant du produit$ 0

Détails du produit

  • Synonyms

    CD3E & CD3D, CD3 delta & CD3 epsilon

  • Source

    APC-Labeled Human CD3E & CD3D Protein, His Tag&Tag Free (CDD-HA2H3) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 & Phe 22 - Ala 105 (Accession # P07766-1 & P04234-1).

    Predicted N-terminus: Asp 23 & Phe 22

  • Molecular Characterization

    The protein has a calculated MW of 29.9 kDa & 13.4 kDa.

  • Conjugate

    APC

    Excitation Wavelength: 640 nm

    Emission Wavelength: 661 nm

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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  • Bioactivity-FACS

     CD3E & CD3D FACS

    1e5 of Mouse Anti-CD3 antibody coupled beads (5.5μm) were stained with different concentration of APC-Labeled Human CD3E & CD3D Protein, His Tag&Tag Free (Cat. No. CDD-HA2H3) and negative control protein respectively, APC signal was used to evaluate the binding activity (QC tested).

    Protocol
  •  CD3E & CD3D FACS

    1e5 of Mouse Anti-CD3 antibody coupled beads (5.5μm) were stained with different concentration of APC-Labeled Human CD3E & CD3D Protein, His Tag&Tag Free (Cat. No. CDD-HA2H3) and negative control protein respectively, APC signal was used to evaluate the binding activity (QC tested).

    Protocol

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Introduction de fond

T-cell surface glycoprotein CD3 delta & CD3 epsilon chain, also known as CD3D & CD3E or CD3D&CD3E respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.

Progrès de frontière

 
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