Cynomolgus CD8 alpha&beta (CD8A&CD8B) Heterodimer Protein, His Tag&Tag Free (MALS verified)

Cat. No. / Size
Price
Qty
CDA-C52W3-100ug
$420.00
CDA-C52W3-1mg
$2720.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    CD8 alpha & beta, CD8A & CD8B

  • Source

    Cynomolgus CD8A&CD8B Heterodimer Protein, His Tag&Tag Free (CDA-C52W3) is expressed from human 293 cells (HEK293). It contains AA Ser 22 - Asp 182 & Leu 22 - Pro 170 (Accession # XP_065382910.1 & XP_005575417.1).

    Predicted N-terminus: Ser 22 & Leu 22

    Request for sequence
  • Molecular Characterization

    CD8 alpha & beta Structure

    Other Tags and Version Biotin & Other Labeled Version

    Cynomolgus CD8A&CD8B Heterodimer Protein, His Tag&Tag Free, produced by co-expression of CD8 alpha and CD8 beta, has a calculated MW of 23.7 kDa (CD8 alpha) and 21.1 kDa (CD8 beta). Subunit CD8 alpha is fused with with a polyhistidine tag at the C-terminus and subunit CD8 beta contains no tag. The protein migrates as 30-35 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 0.1 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    CD8 alpha & beta SDS-PAGE

    Cynomolgus CD8A&CD8B Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    CD8 alpha & beta SEC-MALS

    The purity of Cynomolgus CD8A&CD8B Heterodimer Protein, His Tag&Tag Free (Cat. No. CDA-C52W3) is more than 85% and the molecular weight of this protein is around 45-70 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     CD8 alpha & beta ELISA

    Immobilized Cynomolgus CD8A&CD8B Heterodimer Protein, His Tag&Tag Free (Cat. No. CDA-C52W3) at 1 μg/mL (100 μL/well) can bind Anti-CD8 alpha Antibody, Human IgG1 with a linear range of 0.06-4 ng/mL (QC tested).

    Protocol

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Background

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. CD8A forms disulfide-linked heterodimers with CD8B at the cell surface. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells. Additionally, CD8B plays a critical role in thymic selection of CD8+ T-cells. A palmitoylation site in the cytoplasmic tail of CD8B chain contributes to partitioning of CD8 into the plasma membrane lipid rafts where signaling proteins are enriched.

Recent Advances

 
Drug Development Progress
  • English Name:

    T-cell surface glycoprotein CD8 beta chain

  • Category:

  • Approved Drugs:

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  • Drugs in Clinical Trials:

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  • Highest Development Stage:

    Preclinical

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