Recombinant Streptavidin Protein (MALS verified)

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STN-N5113-5mg
$210.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    Streptavidin, SA

  • Source

    Recombinant Streptavidin Protein (STN-N5113) is expressed from E. coli cells. It contains AA Ala 37 - Ser 163 (Accession # P22629-1).

    Predicted N-terminus: Met

    Request for sequence
  • Molecular Characterization

    Streptavidin Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries no "tag".

    The protein has a calculated MW of 13.4 kDa. The protein migrates as 11-12 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

    The protein is designed as a tetramer.

  • Endotoxin

    Less than 0.1 EU per μg by the LAL method / rFC method.

  • Sterility

    Negative

  • Purity

    >90% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 0.02% NaCl, pH7.3.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    Streptavidin SDS-PAGE

    Recombinant Streptavidin Protein on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    Streptavidin SEC-MALS

    The purity of Recombinant Streptavidin Protein (Cat. No. STN-N5113) is more than 95% and the molecular weight of this protein is around 45-65 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     Streptavidin ELISA

    Immobilized Recombinant Streptavidin Protein (Cat. No. STN-N5113) can bind Biotinylated Human PVRIG, Fc,Avitag (Cat. No. PVG-H82F4) at 1 μg/mL (100 μL/well) with a linear range of 0.2-6 ng/mL (QC tested).

    Protocol

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Background

The Streptavidin Protein Europium chelate is a universal tool for TR-FRET assays that can bind biotinylated molecules. This product uses high-purity streptavidin (SA) covalently conjugated with Eu³⁺ chelate, and it can be used in combination with other Acceptors directly or indirectly labeled with fluorescent dyes. When the Donor and Acceptor come into close proximity (within a distance of less than 10 nm), a FRET reaction occurs: the 620 nm signal emitted by the Donor upon excitation by a specific light source is received by the Acceptor, which then emits a 665 nm signal.

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