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Détails du produit
Synonyms
VEGFC, Flt4-L, VRP
Source
Biotinylated Mouse VEGF-C Protein, His,Avitag (VEC-M82E3) is expressed from human 293 cells (HEK293). It contains AA Ala 108 - Arg 223 (Accession # P97953).
Predicted N-terminus: Ala 108
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 16.6 kDa. The protein migrates as 24-27 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a dimer.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Affichage des données
SDS-PAGE
Biotinylated Mouse VEGF-C Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Mouse VEGF-C Protein, His,Avitag (Cat. No. VEC-M82E3) is more than 90% and the molecular weight of this protein is around 45-65 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Biotinylated Mouse VEGF-C Protein, His,Avitag (Cat. No. VEC-M82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Mouse VEGF R2 Protein, Mouse IgG2a Fc Tag (Cat. No. VE2-M5258) with a linear range of 0.6-10 ng/mL (QC tested).
Protocol
Bioactivity-SPR
Biotinylated Mouse VEGF-C Protein, His,Avitag (Cat. No. VEC-M82E3) immobilized on CM5 Chip can bind Mouse Neuropilin-1, His Tag (Cat. No. NR1-M52H3) with an affinity constant of 4.45 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Biotinylated Mouse VEGF-C Protein, His,Avitag (Cat. No. VEC-M82E3) immobilized on CM5 Chip can bind Human Neuropilin-2, His Tag (Cat. No. NR2-H52H3) with an affinity constant of 7.72 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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Introduction de fond
Progrès de frontière
Progrès du développement médicamenteux- Nom anglais：
Vascular endothelial growth factor C
- Catégorie：
- Nombre de médicaments répertoriés：
0 Détails
- Nombre de médicaments cliniques：
2 Détails
- Étape de développement la plus élevée：
Phase 2 Clinical
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